Computational fluid dynamic analysis of bioprinted self-supporting perfused tissue models.
3D-bioprinting
biofabrication
bioreactor
computational fluid dynamics
perfusion
scaffold-free
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
07
08
2019
revised:
12
11
2019
accepted:
22
11
2019
pubmed:
4
12
2019
medline:
23
2
2021
entrez:
3
12
2019
Statut:
ppublish
Résumé
Natural tissues are incorporated with vasculature, which is further integrated with a cardiovascular system responsible for driving perfusion of nutrient-rich oxygenated blood through the vasculature to support cell metabolism within most cell-dense tissues. Since scaffold-free biofabricated tissues being developed into clinical implants, research models, and pharmaceutical testing platforms should similarly exhibit perfused tissue-like structures, we generated a generalizable biofabrication method resulting in self-supporting perfused (SSuPer) tissue constructs incorporated with perfusible microchannels and integrated with the modular FABRICA perfusion bioreactor. As proof of concept, we perfused an MLO-A5 osteoblast-based SSuPer tissue in the FABRICA. Although our resulting SSuPer tissue replicated vascularization and perfusion observed in situ, supported its own weight, and stained positively for mineral using Von Kossa staining, our in vitro results indicated that computational fluid dynamics (CFD) should be used to drive future construct design and flow application before further tissue biofabrication and perfusion. We built a CFD model of the SSuPer tissue integrated in the FABRICA and analyzed flow characteristics (net force, pressure distribution, shear stress, and oxygen distribution) through five SSuPer tissue microchannel patterns in two flow directions and at increasing flow rates. Important flow parameters include flow direction, fully developed flow, and tissue microchannel diameters matched and aligned with bioreactor flow channels. We observed that the SSuPer tissue platform is capable of providing direct perfusion to tissue constructs and proper culture conditions (oxygenation, with controllable shear and flow rates), indicating that our approach can be used to biofabricate tissue representing primary tissues and that we can model the system in silico.
Identifiants
pubmed: 31788785
doi: 10.1002/bit.27238
pmc: PMC7015804
mid: NIHMS1062459
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
798-815Subventions
Organisme : Indiana Clinical and Translational Sciences Institute
ID : VFR-457-Ekser
Pays : International
Organisme : NCATS NIH HHS
ID : UL1 TR001108
Pays : United States
Organisme : Indiana University Department of Radiology and Imaging Sciences
Pays : International
Organisme : Indiana Center for Musculoskeletal Health
Pays : International
Organisme : NIH HHS
ID : S10 OD023595
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1TR001108
Pays : United States
Informations de copyright
© 2019 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.
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