The impact of intercellular communication for the generation of complex multicellular prevascularized tissue equivalents.
Cell Communication
Cells, Cultured
Coculture Techniques
/ methods
Endothelial Cells
/ cytology
Fibroblast Growth Factor 2
/ metabolism
Fibroblasts
/ cytology
Humans
Interleukin-8
/ metabolism
Microcirculation
Neovascularization, Physiologic
Tissue Engineering
/ methods
Vascular Endothelial Growth Factor A
/ metabolism
HDMEC
angiogenic growth factors
coculture
intercellular communication
microvascular endothelial cells
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
01
09
2019
revised:
21
11
2019
accepted:
22
11
2019
pubmed:
4
12
2019
medline:
1
9
2021
entrez:
3
12
2019
Statut:
ppublish
Résumé
In reconstructive surgery the use of prevascularized soft tissue equivalents is a promising approach for wound coverage of defects after tumor resection or trauma. However, in previous studies to generate soft tissue equivalents on collagen membranes, microcapillaries were restricted to superficial areas. In this study, to understand which factors were involved in the formation of these microcapillaries, the levels of the angiogenic factors vascular endothelial growth factor (VEGF), Interleukin-8 (IL-8), and basic fibroblast growth factor (bFGF) in the supernatants of the tissue equivalents were examined at various time points and conditions. Additionally, the influence of these factors on viability, proliferation, migration, and tube formation in monocultures compared to cocultures of fibroblast and endothelial cells was examined. The results showed that VEGF production was decreased in cocultures compared to fibroblast monocultures and the lowest VEGF levels were observed in endothelial cell monocultures. Additionally, the highest levels of IL-8 were observed in cocultures compared to monocultures. Similar results were observed for bFGF with lowest levels seen within the first 24 hr and highest levels in cocultures. VEGF and IL-8 were shown to promote endothelial cell viability, proliferation and migration and angiogenic parameters such as tube density, total tube length, and number of tube branches. Addition of VEGF and IL-8 to cocultures resulted in accelerated and denser formation of capillary-like structures. The results indicate that VEGF, IL-8, and bFGF strongly influence cellular behavior of endothelial cells and this information should be useful in promoting the formation of microcapillary-like structures in complex tissue equivalents.
Substances chimiques
Interleukin-8
0
Vascular Endothelial Growth Factor A
0
Fibroblast Growth Factor 2
103107-01-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
734-748Informations de copyright
© 2019 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc.
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