DNA methylation is associated with lung function in never smokers.
Adult
Cohort Studies
CpG Islands
/ genetics
DNA Methylation
/ genetics
Epigenesis, Genetic
/ genetics
Female
Forced Expiratory Volume
/ genetics
Genome-Wide Association Study
/ methods
Humans
Male
Middle Aged
Pulmonary Disease, Chronic Obstructive
/ genetics
Reference Values
Smokers
Smoking
/ genetics
COPD
DNA methylation
EWAS
FEV1/FVC
Never smokers
Journal
Respiratory research
ISSN: 1465-993X
Titre abrégé: Respir Res
Pays: England
ID NLM: 101090633
Informations de publication
Date de publication:
02 Dec 2019
02 Dec 2019
Historique:
received:
09
07
2019
accepted:
22
10
2019
entrez:
4
12
2019
pubmed:
4
12
2019
medline:
6
5
2020
Statut:
epublish
Résumé
Active smoking is the main risk factor for COPD. Here, epigenetic mechanisms may play a role, since cigarette smoking is associated with differential DNA methylation in whole blood. So far, it is unclear whether epigenetics also play a role in subjects with COPD who never smoked. Therefore, we aimed to identify differential DNA methylation associated with lung function in never smokers. We determined epigenome-wide DNA methylation levels of 396,243 CpG-sites (Illumina 450 K) in blood of never smokers in four independent cohorts, LifeLines COPD&C (N = 903), LifeLines DEEP (N = 166), Rotterdam Study (RS)-III (N = 150) and RS-BIOS (N = 206). We meta-analyzed the cohort-specific methylation results to identify differentially methylated CpG-sites with FEV A total of 36 CpG-sites were associated with FEV With the identification of 35 CpG-sites that are unique for never smokers, our study shows that DNA methylation is also associated with FEV
Sections du résumé
BACKGROUND
BACKGROUND
Active smoking is the main risk factor for COPD. Here, epigenetic mechanisms may play a role, since cigarette smoking is associated with differential DNA methylation in whole blood. So far, it is unclear whether epigenetics also play a role in subjects with COPD who never smoked. Therefore, we aimed to identify differential DNA methylation associated with lung function in never smokers.
METHODS
METHODS
We determined epigenome-wide DNA methylation levels of 396,243 CpG-sites (Illumina 450 K) in blood of never smokers in four independent cohorts, LifeLines COPD&C (N = 903), LifeLines DEEP (N = 166), Rotterdam Study (RS)-III (N = 150) and RS-BIOS (N = 206). We meta-analyzed the cohort-specific methylation results to identify differentially methylated CpG-sites with FEV
RESULTS
RESULTS
A total of 36 CpG-sites were associated with FEV
CONCLUSIONS
CONCLUSIONS
With the identification of 35 CpG-sites that are unique for never smokers, our study shows that DNA methylation is also associated with FEV
Identifiants
pubmed: 31791327
doi: 10.1186/s12931-019-1222-8
pii: 10.1186/s12931-019-1222-8
pmc: PMC6889726
doi:
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
268Subventions
Organisme : Longfonds
ID : 4.1.13.007
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