Methods for In Vitro Mutagenesis.

Animals Drosophila / genetics Molecular Biology / methods Mutagenesis / genetics Oligonucleotides / genetics

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
02 12 2019
Historique:
entrez: 4 12 2019
pubmed: 4 12 2019
medline: 18 9 2020
Statut: epublish

Résumé

Several different methodologies for mutagenesis have been developed to introduce mutations at predetermined sites or regions within mammalian genes. These methods of in vitro mutagenesis have had a transforming effect on the understanding of functions of protein, transcription regulatory elements, and noncoding RNAs and are now integral to molecular biology investigations. In this introduction, we summarize the most commonly used experimental approaches for mutagenesis and their major applications.

Identifiants

DOI: 10.1101/pdb.top097733 PMID: 31792145
pubmed: 31792145
pii: 2019/12/pdb.top097733
doi: 10.1101/pdb.top097733
doi:

Substances chimiques

Oligonucleotides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2019 Cold Spring Harbor Laboratory Press.

Auteurs

Matteo Forloni (M)

Alex Y Liu (AY)

Narendra Wajapeyee (N)

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Methods for In Vitro Mutagenesis.
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Methods for In Vitro Mutagenesis.
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Biologie moléculaire › Methods for In Vitro Mutagenesis.
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Methods for In Vitro Mutagenesis.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Methods for In Vitro Mutagenesis.