Adaptation to inflammatory acidity through neutrophil-derived adenosine regulation of SLC26A3.
Acidosis
/ chemically induced
Adaptation, Physiological
Adenosine
/ metabolism
Animals
Antiporters
/ genetics
Cells, Cultured
Colitis
/ chemically induced
Disease Models, Animal
Humans
Immune System Diseases
Inflammation
/ chemically induced
Intestinal Mucosa
/ physiology
Intestines
/ immunology
Leukocyte Disorders
Mice
Neutrophil Activation
Neutrophils
/ immunology
Sodium Dodecyl Sulfate
Sulfate Transporters
/ genetics
Transendothelial and Transepithelial Migration
Up-Regulation
Journal
Mucosal immunology
ISSN: 1935-3456
Titre abrégé: Mucosal Immunol
Pays: United States
ID NLM: 101299742
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
11
06
2019
accepted:
07
11
2019
pubmed:
4
12
2019
medline:
20
1
2021
entrez:
4
12
2019
Statut:
ppublish
Résumé
Acute intestinal inflammation includes the early accumulation of neutrophils (PMN). Based on recent evidence that PMN infiltration "imprints" changes in the local tissue environment through local oxygen depletion and the release of adenine nucleotides, we hypothesized that the interaction between transmigrating PMN and intestinal epithelial cells (IECs) results in inflammatory acidification of the tissue. Using newly developed tools, we revealed that active PMN transepithelial migration (TEM) significantly acidifies the local microenvironment, a decrease of nearly 2 pH units. Using unbiased approaches, we sought to define acid-adaptive pathways elicited by PMN TEM. Given the significant amount of adenosine (Ado) generated during PMN TEM, we profiled the influence of Ado on IECs gene expression by microarray and identified the induction of SLC26A3, the major apical Cl
Identifiants
pubmed: 31792360
doi: 10.1038/s41385-019-0237-2
pii: S1933-0219(22)00289-6
pmc: PMC7044055
mid: NIHMS1543192
doi:
Substances chimiques
Antiporters
0
Slc26a3 protein, mouse
0
Sulfate Transporters
0
Sodium Dodecyl Sulfate
368GB5141J
Adenosine
K72T3FS567
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
230-244Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK048520
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK050189
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007038
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK104713
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK103712
Pays : United States
Organisme : BLRD VA
ID : I01 BX002182
Pays : United States
Organisme : NIDDK NIH HHS
ID : R29 DK050189
Pays : United States
Organisme : NIDDK NIH HHS
ID : R37 DK050189
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK095491
Pays : United States
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