Orthogonal regulation of DNA nanostructure self-assembly and disassembly using antibodies.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 12 2019
03 12 2019
Historique:
received:
24
05
2019
accepted:
16
10
2019
entrez:
5
12
2019
pubmed:
5
12
2019
medline:
29
2
2020
Statut:
epublish
Résumé
Here we report a rational strategy to orthogonally control assembly and disassembly of DNA-based nanostructures using specific IgG antibodies as molecular inputs. We first demonstrate that the binding of a specific antibody to a pair of antigen-conjugated split DNA input-strands induces their co-localization and reconstitution into a functional unit that is able to initiate a toehold strand displacement reaction. The effect is rapid and specific and can be extended to different antibodies with the expedient of changing the recognition elements attached to the two split DNA input-strands. Such an antibody-regulated DNA-based circuit has then been employed to control the assembly and disassembly of DNA tubular structures using specific antibodies as inputs. For example, we demonstrate that we can induce self-assembly and disassembly of two distinct DNA tubular structures by using DNA circuits controlled by two different IgG antibodies (anti-Dig and anti-DNP antibodies) in the same solution in an orthogonal way.
Identifiants
pubmed: 31796740
doi: 10.1038/s41467-019-13104-6
pii: 10.1038/s41467-019-13104-6
pmc: PMC6890650
doi:
Substances chimiques
Antibodies
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5509Commentaires et corrections
Type : ErratumIn
Références
Science. 2009 Aug 7;325(5941):725-30
pubmed: 19661424
Nat Chem. 2016 Apr;8(4):368-76
pubmed: 27001733
Nat Chem. 2019 Jun;11(6):510-520
pubmed: 31011170
ACS Nano. 2019 Mar 26;13(3):3545-3554
pubmed: 30835439
Anal Chem. 2019 Jan 2;91(1):44-59
pubmed: 30501170
Nucleic Acids Res. 2016 Aug 19;44(14):e121
pubmed: 27207880
Nat Commun. 2013;4:1965
pubmed: 23756381
J Am Chem Soc. 2006 Oct 4;128(39):12664-5
pubmed: 17002357
Mol Pharmacol. 1987 Jul;32(1):119-26
pubmed: 3600612
Science. 2015 Mar 27;347(6229):1446-52
pubmed: 25814577
Nano Lett. 2011 Jul 13;11(7):2997-3002
pubmed: 21682348
J Am Chem Soc. 2017 Aug 30;139(34):12027-12034
pubmed: 28783358
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10340-4
pubmed: 7937952
Adv Immunol. 1989;44:93-151
pubmed: 2646863
Sci Rep. 2015 May 05;5:9803
pubmed: 25940394
Development. 2018 Jun 26;145(12):
pubmed: 29945988
J Am Chem Soc. 2004 Dec 22;126(50):16344-52
pubmed: 15600335
Nature. 2015 Jul 23;523(7561):441-4
pubmed: 26201596
Cell. 2016 May 19;165(5):1255-1266
pubmed: 27160350
Nano Lett. 2017 Dec 13;17(12):7283-7288
pubmed: 29182337
Anal Chem. 2012 Jan 17;84(2):487-515
pubmed: 22221172
Adv Mater. 2018 Jun;30(24):e1703721
pubmed: 29363798
Clin Cancer Res. 2016 Apr 15;22(8):1845-55
pubmed: 27084738
Nat Commun. 2017 May 08;8:15150
pubmed: 28480878
Angew Chem Int Ed Engl. 2018 Apr 16;57(17):4783-4786
pubmed: 29469991
Nat Chem. 2011 Feb;3(2):103-13
pubmed: 21258382
Cell. 2014 Nov 6;159(4):940-54
pubmed: 25417167
Nat Rev Cancer. 2016 Aug 23;16(9):566-81
pubmed: 27550819
Chem Commun (Camb). 2019 Apr 9;55(30):4387-4390
pubmed: 30916085
Science. 2017 Mar 24;355(6331):
pubmed: 28336611
J Am Chem Soc. 2004 Dec 22;126(50):16342-3
pubmed: 15600334
Science. 2003 Sep 26;301(5641):1882-4
pubmed: 14512621
Nanoscale. 2014 Dec 21;6(24):14919-25
pubmed: 25363245
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):15985-90
pubmed: 24043836
Nature. 2006 Mar 16;440(7082):297-302
pubmed: 16541064
Science. 2011 Jun 3;332(6034):1196-201
pubmed: 21636773
Biochemistry. 1993 Apr 6;32(13):3211-20
pubmed: 8461289
Nat Nanotechnol. 2015 Sep;10(9):748-60
pubmed: 26329111
Nat Chem. 2011 Jul 10;3(8):620-7
pubmed: 21778982
J Am Chem Soc. 2014 Sep 17;136(37):12987-97
pubmed: 25140890
J Am Chem Soc. 2009 Dec 2;131(47):17303-14
pubmed: 19894722
Nat Rev Cancer. 2014 Feb;14(2):135-46
pubmed: 24457417
Biochemistry. 1980 Mar 18;19(6):1182-92
pubmed: 7189409
Chem Commun (Camb). 2017 Jun 29;53(53):7393-7396
pubmed: 28617516
Nat Commun. 2017 Feb 17;8:14473
pubmed: 28211541
Curr Opin Biotechnol. 2019 Aug;58:72-80
pubmed: 30594098
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7015-8
pubmed: 8394005
Nature. 1998 Aug 6;394(6693):539-44
pubmed: 9707114
Curr Opin Chem Biol. 2011 Dec;15(6):789-97
pubmed: 22070901
J Am Chem Soc. 2014 Aug 13;136(32):11198-211
pubmed: 25029570
J Am Chem Soc. 2018 Jan 24;140(3):947-953
pubmed: 29313682