High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure.
X-ray free-electron lasers
microcrystal injection
room-temperature crystallography
serial femtosecond crystallography
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Dec 2019
01 Dec 2019
Historique:
received:
04
05
2019
accepted:
16
09
2019
entrez:
5
12
2019
pubmed:
5
12
2019
medline:
5
12
2019
Statut:
epublish
Résumé
A sample-injection device has been developed at SPring-8 Angstrom Compact Free-Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge-type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature-regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A
Identifiants
pubmed: 31798359
doi: 10.1107/S1600576719012846
pii: te5044
pmc: PMC6878880
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1280-1288Informations de copyright
© Yoshiaki Shimazu et al. 2019.
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