Successful sample preparation for serial crystallography experiments.
XFELs
batch crystallization
micro-crystallization
serial macromolecular crystallography
vapour diffusion
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Dec 2019
01 Dec 2019
Historique:
received:
14
06
2019
accepted:
02
10
2019
entrez:
5
12
2019
pubmed:
5
12
2019
medline:
5
12
2019
Statut:
epublish
Résumé
Serial crystallography, at both synchrotron and X-ray free-electron laser light sources, is becoming increasingly popular. However, the tools in the majority of crystallization laboratories are focused on producing large single crystals by vapour diffusion that fit the cryo-cooled paradigm of modern synchrotron crystallography. This paper presents several case studies and some ideas and strategies on how to perform the conversion from a single crystal grown by vapour diffusion to the many thousands of micro-crystals required for modern serial crystallography grown by batch crystallization. These case studies aim to show (i) how vapour diffusion conditions can be converted into batch by optimizing the length of time crystals take to appear; (ii) how an understanding of the crystallization phase diagram can act as a guide when designing batch crystallization protocols; and (iii) an accessible methodology when attempting to scale batch conditions to larger volumes. These methods are needed to minimize the sample preparation gap between standard rotation crystallography and dedicated serial laboratories, ultimately making serial crystallography more accessible to all crystallographers.
Identifiants
pubmed: 31798361
doi: 10.1107/S1600576719013517
pii: jo5052
pmc: PMC6878878
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1385-1396Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P000622/1
Pays : United Kingdom
Informations de copyright
© John H. Beale et al. 2019.
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