Increased protein intake affects pro-opiomelanocortin (POMC) processing, immune function and IGF signaling in peripheral blood mononuclear cells of home-dwelling old subjects using a genome-wide gene expression approach.
Dairy protein
IGF-1
Immune function
Older adults
POMC
Peripheral mononuclear cells
Randomized Controlled trial
Transcriptomics
Journal
Genes & nutrition
ISSN: 1555-8932
Titre abrégé: Genes Nutr
Pays: Germany
ID NLM: 101280108
Informations de publication
Date de publication:
2019
2019
Historique:
received:
14
03
2019
accepted:
02
10
2019
entrez:
5
12
2019
pubmed:
5
12
2019
medline:
5
12
2019
Statut:
epublish
Résumé
Adequate protein intake among older adults is associated with better health outcomes such as immune function and metabolic regulation of skeletal muscle, but conflicting results make it difficult to define the optimal intake. To further understand the impact of protein intake on metabolic processes, the aim of the study was to explore genome-wide gene expression changes in peripheral blood mononuclear cells (PBMCs) in home-dwelling old subjects after increased protein intake for 12 weeks. In a parallel double-blind randomized controlled intervention study, subjects (≥ 70 years) received a protein-enriched milk (2 × 20 g protein/day, In total 758 gene transcripts were regulated after increased protein intake, and 649 gene transcripts were regulated after intake of carbohydrates ( PBMCs can be used to study gene expression changes after long-term protein intake, as many signaling pathways were regulated after increased protein intake. The functional significance of these findings needs to be further investigated. ClinicalTrials.gov, ID no. NCT02218333. The study was registered on August 18, 2014.
Sections du résumé
BACKGROUND
BACKGROUND
Adequate protein intake among older adults is associated with better health outcomes such as immune function and metabolic regulation of skeletal muscle, but conflicting results make it difficult to define the optimal intake. To further understand the impact of protein intake on metabolic processes, the aim of the study was to explore genome-wide gene expression changes in peripheral blood mononuclear cells (PBMCs) in home-dwelling old subjects after increased protein intake for 12 weeks.
METHOD
METHODS
In a parallel double-blind randomized controlled intervention study, subjects (≥ 70 years) received a protein-enriched milk (2 × 20 g protein/day,
RESULTS
RESULTS
In total 758 gene transcripts were regulated after increased protein intake, and 649 gene transcripts were regulated after intake of carbohydrates (
CONCLUSIONS
CONCLUSIONS
PBMCs can be used to study gene expression changes after long-term protein intake, as many signaling pathways were regulated after increased protein intake. The functional significance of these findings needs to be further investigated.
TRIAL REGISTRATION
BACKGROUND
ClinicalTrials.gov, ID no. NCT02218333. The study was registered on August 18, 2014.
Identifiants
pubmed: 31798754
doi: 10.1186/s12263-019-0654-6
pii: 654
pmc: PMC6883584
doi:
Banques de données
ClinicalTrials.gov
['NCT02218333']
Types de publication
Journal Article
Langues
eng
Pagination
32Informations de copyright
© The Author(s) 2019.
Déclaration de conflit d'intérêts
Competing interestsThe protein-enriched milk and the isocaloric carbohydrate drink were provided by TINE SA, Oslo, Norway, where G.O.G. is employed. She has no financial interest to declare. K.B.H. and S.M.U have received funding from TINE SA. A.R, A.F, I.O, M.M, K.K, S.J.M, K.H.H report no conflict of interest. K.B.H. has received research grant from Mills DA, Olympic Seafood, Amgen, Sanofi, Kaneka and Pronova. S.M.U. has received research grant from Mills DA and Olympic Seafood. None of these grants or honoraria is related to the contents of this manuscript.
Références
Biogerontology. 2013 Jun;14(3):303-23
pubmed: 23686362
Am J Clin Nutr. 2005 May;81(5):1168-75
pubmed: 15883444
J Nutr. 2000 Nov;130(11):2630-5
pubmed: 11053498
Nutrients. 2013 Oct 21;5(10):4184-205
pubmed: 24152751
Int J Mol Sci. 2019 Mar 19;20(6):null
pubmed: 30893897
Ageing Res Rev. 2011 Jul;10(3):319-29
pubmed: 21145432
Eur J Pharmacol. 2011 Jun 11;660(1):213-9
pubmed: 21208604
Eur Heart J. 2008 Nov;29(22):2701-2
pubmed: 18931058
Cancer Epidemiol Biomarkers Prev. 2005 May;14(5):1336-9
pubmed: 15894699
Aging Cell. 2015 Aug;14(4):511-23
pubmed: 25866088
Am J Clin Nutr. 2008 Jan;87(1):150-5
pubmed: 18175749
Nutrients. 2016 Jun 08;8(6):
pubmed: 27338461
J Nutr Health Aging. 2017;21(10):1160-1169
pubmed: 29188875
Mol Nutr Food Res. 2014 Aug;58(8):1708-20
pubmed: 24449395
J Am Med Dir Assoc. 2013 Aug;14(8):542-59
pubmed: 23867520
Nutr Rev. 2007 Dec;65(12 Pt 2):S173-6
pubmed: 18240544
J Nutr Health Aging. 2014 May;18(5):547-53
pubmed: 24886743
Physiol Behav. 2007 Oct 22;92(3):454-60
pubmed: 17512959
Med Sci Sports Exerc. 2010 Dec;42(12):2189-96
pubmed: 20421832
BMJ. 1997 Nov 15;315(7118):1255-60
pubmed: 9390050
Am J Physiol Endocrinol Metab. 2003 Mar;284(3):E481-7
pubmed: 12419777
Br J Nutr. 2011 Dec;106(12):1826-35
pubmed: 21736782
J Nutr. 2014 Jun;144(6):876-80
pubmed: 24477298
J Am Diet Assoc. 1999 Oct;99(10):1228-33
pubmed: 10524386
Nutr Metab Cardiovasc Dis. 2008 Feb;18(2):158-68
pubmed: 18061414
Mol Nutr Food Res. 2012 Jul;56(7):1160-72
pubmed: 22610960
Mech Ageing Dev. 2017 Mar;162:1-8
pubmed: 28163108
Cell Metab. 2014 Mar 4;19(3):407-17
pubmed: 24606898
J Neuroendocrinol. 2008 Jun;20(6):692-9
pubmed: 18601691
Adv Nutr. 2012 May 01;3(3):322-9
pubmed: 22585905
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
PLoS One. 2017 Oct 5;12(10):e0185927
pubmed: 28982156
J Am Med Dir Assoc. 2015 Jan;16(1):85.e1-8
pubmed: 25458445
Clin Chem Lab Med. 2007;45(9):1154-68
pubmed: 17663631
J Psychiatr Res. 2010 Oct;44(13):827-33
pubmed: 20176366
J Am Diet Assoc. 2006 Apr;106(4):569-76
pubmed: 16567153
Annu Rev Physiol. 2004;66:799-828
pubmed: 14977422
Nat Rev Immunol. 2003 Apr;3(4):304-16
pubmed: 12669021
J Am Med Dir Assoc. 2018 Jan;19(1):33-39
pubmed: 29174562
J Nutr. 2007 Aug;137(8):1835-43
pubmed: 17634251
Curr Opin Clin Nutr Metab Care. 2001 Sep;4(5):439-43
pubmed: 11568507
Eur J Clin Nutr. 2013 May;67(5):455-61
pubmed: 23636122
J Gerontol A Biol Sci Med Sci. 2009 Nov;64(11):1183-9
pubmed: 19622801
Nutr Rev. 2007 Dec;65(12 Pt 2):S208-12
pubmed: 18240550
Br J Nutr. 2013 Aug;110 Suppl 2:S1-30
pubmed: 23902657
Maturitas. 2013 May;75(1):51-61
pubmed: 23523272