Geometrical-optics approach to measure the optical density of bacterial cultures using a LED-based photometer.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Nov 2019
01 Nov 2019
Historique:
received:
05
07
2019
revised:
09
09
2019
accepted:
02
10
2019
entrez:
5
12
2019
pubmed:
5
12
2019
medline:
5
12
2019
Statut:
epublish
Résumé
We develop a suitable geometrical-optics approach and demonstrate that it is possible to measure the optical density (OD) of bacterial cultures using a light emitting diode (LED)-based photometer. We measure both attenuation and spot-size variation, and we compensate for diffraction and stray-light impairment related to the incoherent source and large detection area. The approach is validated for different concentrations of two bacterial species,
Identifiants
pubmed: 31799033
doi: 10.1364/BOE.10.005600
pii: 371994
pmc: PMC6865109
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5600-5610Informations de copyright
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The Authors declare that there are no conflicts of interest related to this article.
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