Dual orexin receptor antagonist induces changes in core body temperature in rats after exercise.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
05 12 2019
Historique:
received: 21 05 2019
accepted: 14 11 2019
entrez: 6 12 2019
pubmed: 6 12 2019
medline: 12 11 2020
Statut: epublish

Résumé

Hypothalamic orexin neurons are involved in various physiological functions, including thermoregulation. The orexinergic system has been considered as a potent mediator of the exercise response. The present study describes how the antagonization of the orexinergic system by a dual orexin receptor antagonist (DORA) modifies the thermoregulatory process during exercise. Core Body Temperature (CBT) and Spontaneous Locomotor Activity (SLA) of 12 male Wistar rats were recorded after either oral administration of DORA (30 mg/kg or 60 mg/kg) or placebo solution, both at rest and in exercise conditions with treadmill running. DORA ingestion decreased SLA for 8 hours (p < 0.001) and CBT for 4 hours (p < 0.01). CBT (°C) response was independent of SLA. The CBT level decreased from the beginning to the end of exercise when orexin receptors were antagonized, with a dose-dependent response (39.09 ± 0.36 and 38.88 ± 0.28 for 30 and 60 mg/kg; p < 0.001) compared to placebo (39.29 ± 0.31; p < 0.001). CBT increased during exercise was also blunted after DORA administration, but without dose effects of DORA. In conclusion, our results favor the role of orexin in the thermoregulation under stress related to exercise conditions.

Identifiants

pubmed: 31804545
doi: 10.1038/s41598-019-54826-3
pii: 10.1038/s41598-019-54826-3
pmc: PMC6895233
doi:

Substances chimiques

Orexin Receptor Antagonists 0
Orexin Receptors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

18432

Commentaires et corrections

Type : ErratumIn

Références

Benarroch, E. E. Thermoregulation: recent concepts and remaining questions. Neurology 69, 1293–1297 (2007).
pubmed: 17875917 doi: 10.1212/01.wnl.0000275537.71623.8e
Morrison, S. F. & Nakamura, K. Central Mechanisms for Thermoregulation. Annu. Rev. Physiol. 81, 285–308 (2019).
pubmed: 30256726 doi: 10.1146/annurev-physiol-020518-114546
Nakamura, K. Central circuitries for body temperature regulation and fever. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301, R1207–R1228 (2011).
pubmed: 21900642 doi: 10.1152/ajpregu.00109.2011
de Lecea, L. et al. The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity. Proc. Natl. Acad. Sci. 95, 322–327 (1998).
pubmed: 9419374 pmcid: 18213 doi: 10.1073/pnas.95.1.322
Sakurai, T. et al. Orexins and Orexin Receptors: A Family of Hypothalamic Neuropeptides and G Protein-Coupled Receptors that Regulate Feeding Behavior. Cell. 92, 573–585 (1998).
pubmed: 9491897 doi: 10.1016/S0092-8674(00)80949-6
Peyron, C. et al. Neurons Containing Hypocretin (Orexin) Project to Multiple Neuronal Systems. J. Neurosci. 18, 9996–10015 (1998).
pubmed: 9822755 pmcid: 6793310 doi: 10.1523/JNEUROSCI.18-23-09996.1998
Morrison, S. F. Central neural pathways for thermoregulation. Front. Biosci. 16, 74 (2011).
pmcid: 3051412 doi: 10.2741/3677
Kuwaki, T. Thermoregulation under pressure: a role for orexin neurons. Temperature 2, 379–391 (2015).
doi: 10.1080/23328940.2015.1066921
Kataoka, N., Hioki, H., Kaneko, T. & Nakamura, K. Psychological Stress Activates a Dorsomedial Hypothalamus-Medullary Raphe Circuit Driving Brown Adipose Tissue Thermogenesis and Hyperthermia. Cell Metab. 20, 346–358 (2014).
pubmed: 24981837 doi: 10.1016/j.cmet.2014.05.018
Tupone, D., Madden, C. J., Cano, G. & Morrison, S. F. An orexinergic projection from perifornical hypothalamus to raphe pallidus increases rat brown adipose tissue thermogenesis. J. Neurosci. 31, 15944–15955 (2011).
pubmed: 22049437 pmcid: 3224674 doi: 10.1523/JNEUROSCI.3909-11.2011
Rusyniak, D. E., Zaretsky, D. V., Zaretskaia, M. V., Durant, P. J. & DiMicco, J. A. The Orexin-1 receptor antagonist SB-334867 decreases sympathetic responses to a moderate dose of methamphetamine and stress. Physiol. Behav. 107, 743–750 (2012).
pubmed: 22361264 pmcid: 3371311 doi: 10.1016/j.physbeh.2012.02.010
Gleeson, M. Temperature regulation during exercise. Int J Sports Med. 19(Suppl 2), S96–99 (1998).
pubmed: 9694408 doi: 10.1055/s-2007-971967
Wendt, D., van Loon, L. J. C. & Lichtenbelt, W. D. V. M. Thermoregulation during exercise in the heat: strategies for maintaining health and performance. Sports Med. 37, 669–682 (2007).
pubmed: 17645370 doi: 10.2165/00007256-200737080-00002
Wu, M.-F., John, J., Maidment, N., Lam, H. A. & Siegel, J. M. Hypocretin release in normal and narcoleptic dogs after food and sleep deprivation, eating, and movement. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283, R1079–1086 (2002).
pubmed: 12376401 doi: 10.1152/ajpregu.00207.2002
Martins, P. J. F. et al. Increased hypocretin-1 (orexin-a) levels in cerebrospinal fluid of rats after short-term forced activity. Regul. Pept. 117, 155–158 (2004).
pubmed: 14749034 doi: 10.1016/j.regpep.2003.10.003
Nakabayashi, M. et al. Orexin-A expression in human peripheral tissues. Mol. Cell. Endocrinol. 205, 43–50 (2003).
pubmed: 12890566 doi: 10.1016/S0303-7207(03)00206-5
Zhang, S. et al. Expression of orexin receptors in the brain and peripheral tissues of the male sheep. Regul. Pept. 124, 81–87 (2005).
pubmed: 15544844 doi: 10.1016/j.regpep.2004.07.010
Tsunematsu, T. & Yamanaka, A. The role of orexin/hypocretin in the central nervous system and peripheral tissues. Vitam. Horm. 89, 19–33 (2012).
pubmed: 22640606 doi: 10.1016/B978-0-12-394623-2.00002-0
Mochizuki, T., Klerman, E. B., Sakurai, T. & Scammell, T. E. Elevated body temperature during sleep in orexin knockout mice. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291, R533–540 (2006).
pubmed: 16556901 doi: 10.1152/ajpregu.00887.2005
Zhang, W. et al. Orexin neurons are indispensable for stress-induced thermogenesis in mice: Orexin neurons in stress-induced hyperthermia. J. Physiol. 588, 4117–4129 (2010).
pubmed: 20807795 pmcid: 3002445 doi: 10.1113/jphysiol.2010.195099
Gotter, A. L. et al. Differential sleep-promoting effects of dual orexin receptor antagonists and GABAA receptor modulators. BMC neurosci. 15, 109 (2014).
pubmed: 25242351 pmcid: 4261741 doi: 10.1186/1471-2202-15-109
Estabrooke, I. V. et al. Fos expression in orexin neurons varies with behavioral state. J. Neurosci. 21, 1656–1662 (2001).
pubmed: 11222656 pmcid: 6762959 doi: 10.1523/JNEUROSCI.21-05-01656.2001
Blouin, A. M. et al. Human hypocretin and melanin-concentrating hormone levels are linked to emotion and social interaction. Nat Commun. 4, 1547 (2013).
pubmed: 23462990 doi: 10.1038/ncomms2461
Mileykovskiy, B. Y., Kiyashchenko, L. I. & Siegel, J. M. Behavioral correlates of activity in identified hypocretin/orexin neurons. Neuron. 46, 787–798 (2005).
pubmed: 15924864 pmcid: 8281334 doi: 10.1016/j.neuron.2005.04.035
Lee, M. G., Hassani, O. K. & Jones, B. E. Discharge of identified orexin/hypocretin neurons across the sleep-waking cycle. J. Neurosci. 25, 6716–6720 (2005).
pubmed: 16014733 pmcid: 6725432 doi: 10.1523/JNEUROSCI.1887-05.2005
Brisbare-Roch, C. et al. Promotion of sleep by targeting the orexin system in rats, dogs and humans. Nat. Med. 13, 150–155 (2007).
pubmed: 17259994 doi: 10.1038/nm1544
Nishino, S. et al. Low cerebrospinal fluid hypocretin (Orexin) and altered energy homeostasis in human narcolepsy. Ann. Neurol. 50, 381–388 (2001).
pubmed: 11558795 doi: 10.1002/ana.1130
Gotter, A. L. et al. The duration of sleep promoting efficacy by dual orexin receptor antagonists is dependent upon receptor occupancy threshold. BMC neurosci. 14, 90 (2013).
pubmed: 23981345 pmcid: 3765993 doi: 10.1186/1471-2202-14-90
Morairty, S. R. et al. Dual Hypocretin Receptor Antagonism Is More Effective for Sleep Promotion than Antagonism of Either Receptor Alone. PLoS One. 7, e39131 (2012).
pubmed: 22768296 pmcid: 3388080 doi: 10.1371/journal.pone.0039131
Winrow, C. J. et al. Promotion of Sleep by Suvorexant—A Novel Dual Orexin Receptor Antagonist. J. Neurogenet. 25, 52–61 (2011).
pubmed: 21473737 doi: 10.3109/01677063.2011.566953
Winrow, C. J. et al. Orexin receptor antagonism prevents transcriptional and behavioral plasticity resulting from stimulant exposure. Neuropharmacology. 58, 185–194 (2010).
pubmed: 19596018 doi: 10.1016/j.neuropharm.2009.07.008
Refinetti, R. & Menaker, M. The circadian rhythm of body temperature. Physiol. Behav. 51, 613–637 (1992).
pubmed: 1523238 doi: 10.1016/0031-9384(92)90188-8
Aschoff, J. & Wever, R. A. The circadian system of man. In Handbook on Behavioral Neurobiology. Biological Rhythms. 311–331 (Plenum, 1981).
Kräuchi, K., Cajochen, C. & Wirz-Justice, A. A relationship between heat loss and sleepiness: effects of postural change and melatonin administration. J. Appl. Physiol. 83, 134–139 (1997).
pubmed: 9216955 doi: 10.1152/jappl.1997.83.1.134
Kräuchi, K., Cajochen, C., Pache, M., Flammer, J. & Wirz‐Justice, A. Thermoregulatory effects of melatonin in relation to sleepiness. Chronobiol. Int. 23, 475–484 (2006).
pubmed: 16687320 doi: 10.1080/07420520500545854
Kuroki, C., Takahashi, Y., Ootsuka, Y., Kanmura, Y. & Kuwaki, T. The Impact of Hypothermia on Emergence from Isoflurane Anesthesia in Orexin Neuron-Ablated Mice. Anesth. Analg. 116, 1001–1005 (2013).
pubmed: 23477964 doi: 10.1213/ANE.0b013e31828842f0
Takahashi, Y. et al. Orexin neurons are indispensable for prostaglandin E
pubmed: 23959674 pmcid: 3853500 doi: 10.1113/jphysiol.2013.261271
Gisolfi, C. V. & Mora, F. What’s so important about a body temperature of 37 °C? In The Hot Brain: Survival, Temperature, and Human Body, 95–119 (2000).
Hasegawa, H. et al. Inhibition of the preoptic area and anterior hypothalamus by tetrodotoxin alters thermoregulatory functions in exercising rats. J. Appl. Physiol 98, 1458–1462 (2005).
pubmed: 15618320 doi: 10.1152/japplphysiol.00916.2004
Mohammed, M., Yanagisawa, M., Blessing, W. & Ootsuka, Y. Attenuated cold defense responses in orexin neuron-ablated rats. Temperature 3, 465–475 (2016).
doi: 10.1080/23328940.2016.1184366
Ogawa, Y. et al. Peripherally administered orexin improves survival of mice with endotoxin shock. eLife. 5 (2016).
Samson, W. K., Gosnell, B., Chang, J. K., Resch, Z. T. & Murphy, T. C. Cardiovascular regulatory actions of the hypocretins in brain. Brain Res. 831, 248–253 (1999).
pubmed: 10412003 doi: 10.1016/S0006-8993(99)01457-2
Shirasaka, T., Kunitake, T., Takasaki, M. & Kannan, H. Neuronal effects of orexins: relevant to sympathetic and cardiovascular functions. Regul. Pept. 104, 91–95 (2002).
pubmed: 11830282 doi: 10.1016/S0167-0115(01)00352-4
Monda, M. et al. Sympathetic and hyperthermic reactions by orexin A: role of cerebral catecholaminergic neurons. Regul. Pept. 139, 39–44 (2007).
pubmed: 17134769 doi: 10.1016/j.regpep.2006.10.002
Yoshimichi, G., Yoshimatsu, H., Masaki, T. & Sakata, T. Orexin-A regulates body temperature in coordination with arousal status. Exp. Biol. Med. (Maywood) 226, 468–476 (2001).
doi: 10.1177/153537020122600513
Cano, G. et al. Anatomical substrates for the central control of sympathetic outflow to interscapular adipose tissue during cold exposure. J. Comp. Neurol. 460, 303–326 (2003).
pubmed: 12692852 doi: 10.1002/cne.10643
Dimicco, J. A. & Zaretsky, D. V. The dorsomedial hypothalamus: a new player in thermoregulation. Am. J. Physiol. Regul. Integr. Comp. Physiol. 292, R47–63 (2007).
pubmed: 16959861 doi: 10.1152/ajpregu.00498.2006
Monda, M., Viggiano, A., Viggiano, A., Fuccio, F. & De Luca, V. Injection of orexin A into the diagonal band of Broca induces sympathetic and hyperthermic reactions. Brain Res. 1018, 265–271 (2004).
pubmed: 15276887 doi: 10.1016/j.brainres.2004.05.084
May, F. J. et al. Lipidomic Adaptations in White and Brown Adipose Tissue in Response to Exercise Demonstrates Molecular Species-Specific Remodeling. Cell Rep. 18, 1558–1572 (2017).
pubmed: 28178530 pmcid: 5558157 doi: 10.1016/j.celrep.2017.01.038
De Matteis, R. et al. Exercise as a new physiological stimulus for brown adipose tissue activity. Nutr Metab Cardiovasc Dis. 23, 582–590 (2013).
pubmed: 22633794 doi: 10.1016/j.numecd.2012.01.013
Lee, P. et al. Irisin and FGF21 Are Cold-Induced Endocrine Activators of Brown Fat Function in Humans. Cell Metab. 19, 302–309 (2014).
pubmed: 24506871 pmcid: 7647184 doi: 10.1016/j.cmet.2013.12.017
Martins, L. et al. A Functional Link between AMPK and Orexin Mediates the Effect of BMP8B on Energy Balance. Cell Rep. 16, 2231–2242 (2016).
pubmed: 27524625 pmcid: 4999418 doi: 10.1016/j.celrep.2016.07.045
Dauvilliers, Y., Arnulf, I. & Mignot, E. Narcolepsy with cataplexy. Lancet. 369, 499–511 (2007).
pubmed: 17292770 doi: 10.1016/S0140-6736(07)60237-2
Dauvilliers, Y., Siegel, J. M., Lopez, R., Torontali, Z. A. & Peever, J. H. Cataplexy–clinical aspects, pathophysiology and management strategy. Nat Rev Neurol. 10, 386–395 (2014).
pubmed: 24890646 pmcid: 8788644 doi: 10.1038/nrneurol.2014.97
Fronczek, R., Overeem, S., Lammers, G. J., van Dijk, J. G. & Van Someren, E. J. W. Altered skin-temperature regulation in narcolepsy relates to sleep propensity. Sleep. 29, 1444–1449 (2006).
pubmed: 17162991 doi: 10.1093/sleep/29.11.1444
Fronczek, R. et al. Manipulation of skin temperature improves nocturnal sleep in narcolepsy. J. Neurol. Neurosurg. Psychiatry. 79, 1354–1357 (2008).
pubmed: 18653548 doi: 10.1136/jnnp.2008.143610
van der Heide, A. et al. The effects of sodium oxybate on core body and skin temperature regulation in narcolepsy. J Sleep Res. 24, 566–575 (2015).
pubmed: 25913575 doi: 10.1111/jsr.12303
Enevoldsen, L. H. et al. Functional brown adipose tissue and sympathetic activity after cold exposure in humans with type 1 narcolepsy. Sleep. 41 (2018).
Dauvilliers, Y., Billiard, M. & Montplaisir, J. Clinical aspects and pathophysiology of narcolepsy. Clin Neurophysiol. 114, 2000–2017 (2003).
pubmed: 14580598 doi: 10.1016/S1388-2457(03)00203-7
Koumar, O., Crunel, V., Fréret, T., Moussay, S. & Bessot, N. Effect of circadian thermoregulatory phase in recovery of both temperature and rest/activity rhythms after a standard physical exercise in rats Koumar, O.-C., Crunel, V., Bouet, V., Fréret, T., Moussay, S. & Bessot, N. (Caen, FR) ESRS Talinn 2014 P571. In P571 (2014).
Martin, T. et al. Vestibular loss disrupts daily rhythm in rats. J. Appl. Physiol. 118, 310–318 (2015).
pubmed: 25505031 doi: 10.1152/japplphysiol.00811.2014
Pinheiro, J. Ć. & Bates, D. M. Mixed Effects Models in S and S-Plus. (Springer, 2000).

Auteurs

Tristan Martin (T)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France.

Yves Dauvilliers (Y)

Reference National Center for Narcolepsy, Sleep Unit, Department of Neurology, Gui-de-Chauliac Hospital, University of Montpellier, Montpellier, INSERM U1061, France.

Ouma-Chandrou Koumar (OC)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France.

Valentine Bouet (V)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France.

Thomas Freret (T)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France.

Stéphane Besnard (S)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France.

François Dauphin (F)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France.

Nicolas Bessot (N)

Normandie Univ, Unicaen, INSERM, COMETE, 14000, Caen, France. nicolas.bessot@unicaen.fr.

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