Studies of blood-brain barrier permeability of gastrodigenin in vitro and in vivo.
Blood–brain barrier
Gastrodia elata
Gastrodigenin
Microdialysis
hCMEC/D3
Journal
Fitoterapia
ISSN: 1873-6971
Titre abrégé: Fitoterapia
Pays: Netherlands
ID NLM: 16930290R
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
04
10
2019
revised:
30
11
2019
accepted:
30
11
2019
pubmed:
6
12
2019
medline:
14
1
2020
entrez:
6
12
2019
Statut:
ppublish
Résumé
According to the basic theories of traditional Chinese medicine, Gastrodia elata (GE) is clinically utilized for the treatment of cephalalgia and migraine. The gastrodigenin (p-hydroxybenzyl alcohol, HBA), one of the effective components of GE, may pass through the blood-brain barrier (BBB) to exert its pharmacological effects. This study aimed to investigate BBB permeability of HBA via in vitro hCMEC/D3 BBB model and in vivo microdialysis in rats. For the establishment of in vitro BBB model, hCMEC/D3 cells were used to construct the monolayer. The integrity of the monolayer was evaluated by TEER measurements, expression analysis of tight junction proteins (claudin-5, zo-1 and occludin) and apparent permeability coefficients (P
Identifiants
pubmed: 31805306
pii: S0367-326X(19)31998-7
doi: 10.1016/j.fitote.2019.104447
pii:
doi:
Substances chimiques
Benzyl Alcohols
0
4-hydroxybenzyl alcohol
1A3AH1FP1B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104447Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.