Leaf status and environmental signals jointly regulate proline metabolism in winter oilseed rape.
Brassica napus
PROLINE DEHYDROGENASE (ProDH)
Δ
1
-PYRROLINE-5-CARBOXYLATE SYNTHASE (P5CS)
fluxes
osmotic stress
proline
regulation
senescence
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
25 03 2020
25 03 2020
Historique:
received:
09
09
2019
accepted:
05
12
2019
pubmed:
7
12
2019
medline:
15
5
2021
entrez:
7
12
2019
Statut:
ppublish
Résumé
Proline metabolism is an essential component of plant adaptation to multiple environmental stress conditions that is also known to participate in specific developmental phases, particularly in reproductive organs. Recent evidence suggested a possible role for proline catabolism in Brassica napus for nitrogen remobilization processes from source leaves at the vegetative stage. Here, we investigate transcript levels of Δ1-PYRROLINE-5-CARBOXYLATE SYNTHASE (P5CS) and PROLINE DEHYDROGENASE (ProDH) genes at the vegetative stage with respect to net proline biosynthesis and degradation fluxes in leaves having a different sink/source balance. We showed that the underexpression of three P5CS1 genes in source leaves was accompanied by a reduced commitment of de novo assimilated 15N towards proline biosynthesis and an overall depletion of free proline content. We found that the expression of ProDH genes was strongly induced by carbon starvation conditions (dark-induced senescence) compared with early senescing leaves. Our results suggested a role for proline catabolism in B. napus, but acting only at a late stage of senescence. In addition, we also identified some P5CS and ProDH genes that were differentially expressed during multiple processes (leaf status, dark to light transition, and stress response).
Identifiants
pubmed: 31807778
pii: 5661025
doi: 10.1093/jxb/erz538
pmc: PMC7242077
doi:
Substances chimiques
Proline
9DLQ4CIU6V
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2098-2111Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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