Lack of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Disturbs Callus Formation.
ALP
BMP
Callus
Collagen type I
PACAP
Tibia fracture
Journal
Journal of molecular neuroscience : MN
ISSN: 1559-1166
Titre abrégé: J Mol Neurosci
Pays: United States
ID NLM: 9002991
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
08
10
2019
accepted:
13
11
2019
pubmed:
7
12
2019
medline:
6
1
2022
entrez:
7
12
2019
Statut:
ppublish
Résumé
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a naturally secreted signaling peptide and has important regulatory roles in the differentiation of the central nervous system and its absence results in disorders in femur development. PACAP has an important function in prevention of oxidative stress or mechanical stress in chondrogenesis but little is known about its function in bone regeneration. A new callus formation model was set to investigate its role in bone remodeling. Fracturing was 5 mm distal from the proximal articular surface of the tibia and the depth was 0.5 mm. Reproducibility of callus formation was investigated with CT 3, 7, and 21 days after the operation. Absence of PACAP did not alter the alkaline phosphatase (ALP) activation in PACAP KO healing process. In developing callus, the expression of collagen type I increased in wild-type (WT) and PACAP KO mice decreased to the end of healing process. Expression of the elements of BMP signaling was disturbed in the callus formation of PACAP KO mice, as bone morphogenic protein 4 (BMP4) and 6 showed an early reduction in bone regeneration. However, elevated Smad1 expression was demonstrated in PACAP KO mice. Our results indicate that PACAP KO mice show various signs of disturbed bone healing and suggest PACAP compensatory and fine tuning effects in proper bone regeneration.
Identifiants
pubmed: 31808034
doi: 10.1007/s12031-019-01448-z
pii: 10.1007/s12031-019-01448-z
pmc: PMC8349325
doi:
Substances chimiques
Bmp4 protein, mouse
0
Bone Morphogenetic Protein 4
0
Collagen Type I
0
Pituitary Adenylate Cyclase-Activating Polypeptide
0
Smad1 Protein
0
Smad1 protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1543-1555Informations de copyright
© 2019. The Author(s).
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