First record and analysis of the COI gene of Cobboldia elephantis obtained from a captive Asian elephant from Myanmar.
Asian elephant
COI gene
Cobboldia elephantis
Myanmar
phylogenetic tree
stomach bot fly
Journal
Parasitology international
ISSN: 1873-0329
Titre abrégé: Parasitol Int
Pays: Netherlands
ID NLM: 9708549
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
17
08
2019
revised:
11
11
2019
accepted:
02
12
2019
pubmed:
7
12
2019
medline:
22
8
2020
entrez:
7
12
2019
Statut:
ppublish
Résumé
The stomach bot fly species in Asian elephants has long been known as Cobboldia elephantis. However, there is no genetic information available for this species to date. Here, we report that a third-instar fly larva was excreted from a captive Asian elephant four months after export from an elephant camp in Myanmar to a zoological garden in Japan. Morphological characteristics of the larva were coincident with published descriptions of C. elephantis. The mitochondrial cytochrome c oxidase subunit I (COI) gene was amplified from the larva by PCR using primers modified from those designed for DNA barcoding of insects and amphibians. The COI gene of C. elephantis showed 76.6 % and 83.6 % identity at the nucleotide and amino acid levels, respectively, to that of C. loxodontis, the stomach bot fly species in African elephants. Phylogenetic analysis of the COI genes of several stomach bot fly species revealed that the two Cobboldia species formed a clade separate from the stomach bot fly species found in rhinoceros and equids.
Identifiants
pubmed: 31809880
pii: S1383-5769(19)30386-1
doi: 10.1016/j.parint.2019.102035
pii:
doi:
Substances chimiques
Insect Proteins
0
Electron Transport Complex IV
EC 1.9.3.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102035Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None.