Establishment of an automated patch-clamp platform for electrophysiological and pharmacological evaluation of hiPSC-CMs.
Automated patch-clamp
Ca(2+) current
Dynamic current-clamp
Human induced pluripotent stem cell-derived cardiomyocyte
Na(+) current
Transient outward and inward rectifier K(+) currents
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
30
04
2019
revised:
05
11
2019
accepted:
17
11
2019
pubmed:
7
12
2019
medline:
3
6
2020
entrez:
7
12
2019
Statut:
ppublish
Résumé
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology. However, the electrophysiological and pharmacological applications of hiPSC-CMs are hampered by manual patch-clamp technique, which is labor-intensive and generates low output. The automated patch-clamp technique is showing potential to overcome this problem. Here, we describe a new dissociation method, with which we can harvest a vast number of single relaxed hiPSC-CMs with smooth membrane suited for automated patch-clamp. Using the automated whole-cell patch-clamp technology, we report a high success rate for cell capture and whole-cell access (around 70%). We are able to identify and record several currents and paced action potentials (APs) with different success rates, including Na
Identifiants
pubmed: 31809994
pii: S1873-5061(19)30292-2
doi: 10.1016/j.scr.2019.101662
pii:
doi:
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101662Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.