Effects of Medium-chain Triglycerides Administration in Chemically-induced Carcinogenesis in Mice.
3-Hydroxybutyric Acid
/ blood
Adipocytes
/ pathology
Adipokines
/ metabolism
Adiponectin
/ metabolism
Adipose Tissue
/ drug effects
Aldehydes
/ metabolism
Animal Feed
/ analysis
Animals
Carcinogens
Carcinoma, Hepatocellular
/ chemically induced
Cell Count
Cell Proliferation
Chemokines
/ metabolism
Cytokines
/ metabolism
Diethylnitrosamine
Hypertrophy
Inflammation
/ drug therapy
Leptin
/ metabolism
Liver
/ metabolism
Liver Neoplasms, Experimental
/ chemically induced
Male
Mice
Mice, Inbred C3H
Oxidative Stress
Triglycerides
/ administration & dosage
Medium-chain triglycerides
adipocytokine
anti-inflammatory effect
antioxidant action
diethylnitrosamine
hepatocellular carcinoma
ketone body
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
16
10
2019
revised:
08
11
2019
accepted:
11
11
2019
entrez:
8
12
2019
pubmed:
8
12
2019
medline:
18
12
2019
Statut:
ppublish
Résumé
The aim of this study was to investigate the effects of medium-chain triglycerides (MCTs) on chemically-induced hepatic carcinogenesis (HCC) in mice. In a first set of experiments, mice were treated with diethylnitrosoamine intraperitoneally at two weeks of age. They were fed chow containing MCT or a normal chow diet and sacrificed after 28 weeks. Incidence of hepatic tumor was compared between the two groups. Expression of oxidative stress, and inflammatory cytokines and chemokines in liver tissues were examined. In a second set of experiments, the histopathological findings of the intraperitoneal adipose tissue were assessed, and expression of adipocytokines in the fat tissue was measured. In a third set of experiments, plasma β-hydroxybutyrate (HB) concentration was measured in both animals fed chow containing MCT and a normal chow diet. Mouse HCC cells were co-cultured with β-HB, and the numbers of tumor cells were counted at days 3 and 7. In the first set of experiments, the tumor count observed in the control group was significantly blunted in the MCT group. Maximum tumor diameter also decreased in the MCT group compared to the control group. The expression of inflammatory cytokines and chemokines was significantly decreased by MCT. Furthermore, expression of 4-hydroxynonenal was lower in the MCT group compared to the control group. In the second set of experiments, hypertrophy of the adipocytes was suppressed, and the concentration of adiponectin and leptin in the adipose tissue decreased by MCT. In the third set of experiments, plasma β-HB concentration increased in the MCT group as expected. β-HB significantly inhibited the proliferation of HCC cells. MCT administration markedly suppresses the incidence of chemically-induced HCC by inhibition of inflammation and increase of ketone bodies.
Identifiants
pubmed: 31810930
pii: 39/12/6653
doi: 10.21873/anticanres.13880
doi:
Substances chimiques
Adipokines
0
Adiponectin
0
Adipoq protein, mouse
0
Aldehydes
0
Carcinogens
0
Chemokines
0
Cytokines
0
Leptin
0
Triglycerides
0
Diethylnitrosamine
3IQ78TTX1A
4-hydroxy-2-nonenal
K1CVM13F96
3-Hydroxybutyric Acid
TZP1275679
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6653-6660Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.