Adenine-based small molecule fluorescent probe for imaging mitochondrial nucleic acid.
Adenine
Cell imaging
DNA
Fluorescence probe
Mitochondria
RNA
Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Titre abrégé: Spectrochim Acta A Mol Biomol Spectrosc
Pays: England
ID NLM: 9602533
Informations de publication
Date de publication:
15 Mar 2020
15 Mar 2020
Historique:
received:
12
11
2019
revised:
26
11
2019
accepted:
26
11
2019
pubmed:
10
12
2019
medline:
21
10
2020
entrez:
10
12
2019
Statut:
ppublish
Résumé
A small molecule fluorescent probe (probe 1) based on adenine-coumarin derivative was designed and synthesized in this paper. Probe 1 exhibited a significant fluorescence-enhancing response to nucleic acids at 495 nm (for DNA) and 487 nm (for RNA). The fluorescence enhancement of probe 1 for DNA and RNA was 5.68 and 9.73 times respectively, the fluorescence quantum yield was changed from 2.5% to 11.7% and 22.5% accordingly. Meanwhile, an excellent linear relationship of fluorescence intensity at 495 nm or 487 nm versus the nucleic acid concentration (1 μM for probe 1, 0-350 μg/mL for DNA and 0-300 μg/mL for RNA) was obtained. Co-staining and nucleic acid digestion experiments showed that probe 1 could selectively image nucleic acids in mitochondria and nucleoli in HeLa cells.
Identifiants
pubmed: 31813730
pii: S1386-1425(19)31255-7
doi: 10.1016/j.saa.2019.117865
pii:
doi:
Substances chimiques
DNA, Mitochondrial
0
Fluorescent Dyes
0
Adenine
JAC85A2161
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117865Informations de copyright
Copyright © 2019. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest There are no conflicts to declare.