Fecal microbiome as determinant of the effect of diet on colorectal cancer risk: comparison of meat-based versus pesco-vegetarian diets (the MeaTIc study).
Colorectal cancer
Diet
Intestinal microbiome
Meat
Neoplastic disease
Prevention
Vegetarian
Journal
Trials
ISSN: 1745-6215
Titre abrégé: Trials
Pays: England
ID NLM: 101263253
Informations de publication
Date de publication:
09 Dec 2019
09 Dec 2019
Historique:
received:
08
08
2019
accepted:
11
10
2019
entrez:
10
12
2019
pubmed:
10
12
2019
medline:
28
7
2020
Statut:
epublish
Résumé
Convincing evidence suggests that the risk of colorectal cancer (CRC) is increased by the typical Western diet characterized by high consumption of red and processed meat. In addition, some epidemiological studies suggest a reduction in the risk of CRC associated with fish consumption. The role of the gut microbiome in this diet-associated risk is not well understood. This is a randomized parallel open clinical trial comprising a total of 150 clinically healthy subjects randomly assigned to three groups: a meat-based diet of which 4 portions per week are red meat (1 portion = 150 g), 3 portions per week are processed meat (1 portion = 50 g), and 1 portion per week is poultry (1 portion = 150 g), for a total amount of 900 g per week of meat and derivatives; a meat-based diet supplemented with alpha-tocopherol; and a pesco-vegetarian diet excluding fresh and processed meat and poultry, but which includes 3 portions per week of fish for a total amount of 450 g per week. Each intervention will last 3 months. The three diets will be isocaloric and of three different sizes according to specific energy requirements. Anthropometric measurements, body composition, and blood and fecal samples will be obtained from each participant at the beginning and end of each intervention phase. The measure of the primary outcome will be the change from baseline in DNA damage induced by fecal water using the comet assay in a cellular model. Secondary outcome measures will be changes in the profile of fecal microbiomes, global fecal and urinary peroxidation markers, and neoplastic biomarkers. Although epidemiological data support the promoting role of meat and the possible protective role of fish in colon carcinogenesis, no study has directly compared dietary profiles characterized by the presence of these two food groups and the role of the gut microbiome in these diet-associated CRC risks. This study will test the effect of these dietary profiles on validated CRC risk biomarkers. ClinicalTrials.gov, NCT03416777. Registered on 3 May 2018.
Sections du résumé
BACKGROUND
BACKGROUND
Convincing evidence suggests that the risk of colorectal cancer (CRC) is increased by the typical Western diet characterized by high consumption of red and processed meat. In addition, some epidemiological studies suggest a reduction in the risk of CRC associated with fish consumption. The role of the gut microbiome in this diet-associated risk is not well understood.
METHODS/DESIGN
METHODS
This is a randomized parallel open clinical trial comprising a total of 150 clinically healthy subjects randomly assigned to three groups: a meat-based diet of which 4 portions per week are red meat (1 portion = 150 g), 3 portions per week are processed meat (1 portion = 50 g), and 1 portion per week is poultry (1 portion = 150 g), for a total amount of 900 g per week of meat and derivatives; a meat-based diet supplemented with alpha-tocopherol; and a pesco-vegetarian diet excluding fresh and processed meat and poultry, but which includes 3 portions per week of fish for a total amount of 450 g per week. Each intervention will last 3 months. The three diets will be isocaloric and of three different sizes according to specific energy requirements. Anthropometric measurements, body composition, and blood and fecal samples will be obtained from each participant at the beginning and end of each intervention phase. The measure of the primary outcome will be the change from baseline in DNA damage induced by fecal water using the comet assay in a cellular model. Secondary outcome measures will be changes in the profile of fecal microbiomes, global fecal and urinary peroxidation markers, and neoplastic biomarkers.
DISCUSSION
CONCLUSIONS
Although epidemiological data support the promoting role of meat and the possible protective role of fish in colon carcinogenesis, no study has directly compared dietary profiles characterized by the presence of these two food groups and the role of the gut microbiome in these diet-associated CRC risks. This study will test the effect of these dietary profiles on validated CRC risk biomarkers.
TRIAL REGISTRATION
BACKGROUND
ClinicalTrials.gov, NCT03416777. Registered on 3 May 2018.
Identifiants
pubmed: 31815647
doi: 10.1186/s13063-019-3801-x
pii: 10.1186/s13063-019-3801-x
pmc: PMC6902610
doi:
Banques de données
ClinicalTrials.gov
['NCT03416777']
Types de publication
Comparative Study
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Pagination
688Références
Cancer Prev Res (Phila). 2010 Jul;3(7):852-64
pubmed: 20530708
Free Radic Biol Med. 2015 Jun;83:192-200
pubmed: 25744414
Am J Clin Nutr. 2013 Nov;98(5):1255-62
pubmed: 24025632
Cancer Res. 2015 Mar 1;75(5):870-9
pubmed: 25592152
Nutr Cancer. 2015;67(1):119-25
pubmed: 25514759
Nature. 2014 Jan 23;505(7484):559-63
pubmed: 24336217
Am J Public Health. 2014 Jun;104 Suppl 3:S404-14
pubmed: 24754657
PLoS Comput Biol. 2015 Mar 27;11(3):e1004186
pubmed: 25815895
Ann Oncol. 2017 Aug 1;28(8):1788-1802
pubmed: 28407090
FASEB J. 1998 Sep;12(12):1063-73
pubmed: 9737710
Microbiome. 2019 May 6;7(1):72
pubmed: 31060614
Trends Mol Med. 2002 Jan;8(1):10-6
pubmed: 11796261
Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14691-6
pubmed: 20679230
Front Microbiol. 2017 Feb 28;8:300
pubmed: 28293225
Oncotarget. 2016 Nov 29;7(48):79008-79016
pubmed: 27738321
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Nat Rev Gastroenterol Hepatol. 2016 Dec;13(12):691-706
pubmed: 27848961
Clin Cancer Res. 2014 Feb 15;20(4):799-803
pubmed: 24270685
Cancer Epidemiol Biomarkers Prev. 2016 Apr;25(4):640-7
pubmed: 26823477
Am J Clin Nutr. 2007 Feb;85(2):488-96
pubmed: 17284748