New approach in radiometabolite analysis of positron emission tomography (PET) radioligands; lead-shielded microextraction by packed sorbent as a tool for in vivo radiometabolite analysis of [
High performance liquid chromatography (HPLC)
Microextraction by packed sorbents (MEPS)
Positron emission tomography (PET)
Protein precipitation (PP)
Radiometabolite analysis (RMA)
Rat plasma
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Feb 2020
01 Feb 2020
Historique:
received:
11
08
2019
revised:
01
10
2019
accepted:
04
10
2019
entrez:
11
12
2019
pubmed:
11
12
2019
medline:
5
6
2020
Statut:
ppublish
Résumé
The blood plasma radiometabolite analysis (RMA) is crucial procedure for quantitative analysis of positron emission tomography (PET) radioligands with no original reference region in the brain. Blood sampling and separating plasma for RMA has been a challenge especially with small animal PET research. This work aimed to overcome this problem by presenting a semi-automated lead shielded microextraction by packed sorbents (SLS-MEPS). The capability of SLS-MEPS was evaluated for RMA of [
Identifiants
pubmed: 31816716
pii: S0039-9140(19)31082-3
doi: 10.1016/j.talanta.2019.120449
pii:
doi:
Substances chimiques
Carbon Radioisotopes
0
Carbon-11
0
Ligands
0
Radiopharmaceuticals
0
Receptors, Purinergic P2X7
0
Lead
2P299V784P
Carbon
7440-44-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
120449Informations de copyright
Copyright © 2019. Published by Elsevier B.V.