ARS2 Regulates Nuclear Paraspeckle Formation through 3'-End Processing and Stability of NEAT1 Long Noncoding RNA.
ARS2
NEAT1
long noncoding RNA
paraspeckle
Journal
Molecular and cellular biology
ISSN: 1098-5549
Titre abrégé: Mol Cell Biol
Pays: United States
ID NLM: 8109087
Informations de publication
Date de publication:
30 01 2020
30 01 2020
Historique:
received:
16
06
2019
accepted:
12
11
2019
pubmed:
11
12
2019
medline:
9
7
2020
entrez:
11
12
2019
Statut:
epublish
Résumé
Nuclear paraspeckle assembly transcript 1 (NEAT1) is a long noncoding RNA that functions as an essential framework of subnuclear paraspeckle bodies. Of the two isoforms (NEAT1_1 and NEAT1_2) produced by alternative 3'-end RNA processing, the longer isoform, NEAT1_2, plays a crucial role in paraspeckle formation. Here, we demonstrate that the 3'-end processing and stability of NEAT1 RNAs are regulated by arsenic resistance protein 2 (ARS2), a factor interacting with the cap-binding complex (CBC) that binds to the m7G cap structure of RNA polymerase II transcripts. The knockdown of ARS2 inhibited the association between NEAT1 and mammalian cleavage factor I (CFIm), which produces the shorter isoform, NEAT1_1. Furthermore, the knockdown of ARS2 led to the preferential stabilization of NEAT1_2. As a result, NEAT1_2 RNA levels were markedly elevated in ARS2 knockdown cells, leading to an increase in the number of paraspeckles. These results reveal a suppressive role for ARS2 in NEAT1_2 expression and the subsequent formation of paraspeckles.
Identifiants
pubmed: 31818879
pii: MCB.00269-19
doi: 10.1128/MCB.00269-19
pmc: PMC6996275
pii:
doi:
Substances chimiques
MALAT1 long non-coding RNA, human
0
NEAT1 long non-coding RNA, human
0
Nuclear Proteins
0
RNA, Long Noncoding
0
RNA, Small Interfering
0
SRRT protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 American Society for Microbiology.
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