Pancreatic cancer organoids recapitulate disease and allow personalized drug screening.

biobank cancer organoids pancreatic cancer personalized medicine

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
26 Dec 2019
Historique:
pubmed: 11 12 2019
medline: 11 12 2019
entrez: 11 12 2019
Statut: ppublish

Résumé

We report the derivation of 30 patient-derived organoid lines (PDOs) from tumors arising in the pancreas and distal bile duct. PDOs recapitulate tumor histology and contain genetic alterations typical of pancreatic cancer. In vitro testing of a panel of 76 therapeutic agents revealed sensitivities currently not exploited in the clinic, and underscores the importance of personalized approaches for effective cancer treatment. The PRMT5 inhibitor EZP015556, shown to target

Identifiants

pubmed: 31818951
pii: 1911273116
doi: 10.1073/pnas.1911273116
pmc: PMC6936689
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

26580-26590

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom

Références

Cancer Genet Cytogenet. 1996 Jan;86(1):22-8
pubmed: 8616780
Nature. 1993 Dec 16;366(6456):704-7
pubmed: 8259215
Cell. 2018 Apr 5;173(2):515-528.e17
pubmed: 29625057
Cancer Cell. 2018 Apr 9;33(4):676-689.e3
pubmed: 29622463
N Engl J Med. 2011 May 12;364(19):1817-25
pubmed: 21561347
Cancer Med. 2016 Jan;5(1):88-99
pubmed: 26645826
Adv Exp Med Biol. 1988;250:165-77
pubmed: 3151224
Genome Med. 2018 Mar 28;10(1):25
pubmed: 29592813
Bioinformatics. 2016 Sep 1;32(17):i413-i420
pubmed: 27587657
Nature. 2016 Oct 13;538(7624):260-264
pubmed: 27698416
Cell. 2018 Apr 5;173(2):371-385.e18
pubmed: 29625053
Cell Rep. 2016 Apr 19;15(3):574-587
pubmed: 27068473
Nature. 2016 Mar 3;531(7592):47-52
pubmed: 26909576
Cancer Discov. 2019 Jul;9(7):852-871
pubmed: 31053628
Science. 2016 Mar 11;351(6278):1214-8
pubmed: 26912360
Am J Pathol. 1995 Sep;147(3):790-8
pubmed: 7677190
Eur J Cancer. 2013 Apr;49(6):1305-13
pubmed: 23265702
Nat Genet. 2015 Dec;47(12):1402-7
pubmed: 26551669
Cell. 2015 Jan 15;160(1-2):324-38
pubmed: 25557080
Science. 2016 Mar 11;351(6278):1208-13
pubmed: 26912361
Cell. 2015 May 7;161(4):933-45
pubmed: 25957691
Nature. 2010 Feb 18;463(7283):899-905
pubmed: 20164920
Nat Med. 2019 May;25(5):838-849
pubmed: 31011202
Cell Stem Cell. 2018 Mar 1;22(3):454-467.e6
pubmed: 29337182
Cancer Discov. 2018 Sep;8(9):1112-1129
pubmed: 29853643
Nat Rev Cancer. 2018 Jul;18(7):407-418
pubmed: 29692415
Trends Biochem Sci. 2011 Dec;36(12):633-41
pubmed: 21975038
Genes Dev. 2012 Jun 15;26(12):1268-86
pubmed: 22713868
Nat Chem Biol. 2015 Jun;11(6):432-7
pubmed: 25915199
Cell. 1997 Nov 28;91(5):649-59
pubmed: 9393858
Nat Commun. 2019 Oct 8;10(1):4571
pubmed: 31594944
Cell. 2018 Jan 11;172(1-2):373-386.e10
pubmed: 29224780
N Engl J Med. 2013 Oct 31;369(18):1691-703
pubmed: 24131140
Genome Res. 2019 Jul;29(7):1067-1077
pubmed: 31221724
J Biol Chem. 2007 Jul 20;282(29):21477-86
pubmed: 17548352
Science. 2018 Feb 23;359(6378):920-926
pubmed: 29472484
J Cell Biochem. 2009 Feb 1;106(2):210-9
pubmed: 19097084
Nat Med. 2017 Apr;23(4):517-525
pubmed: 28288110

Auteurs

Else Driehuis (E)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands.

Arne van Hoeck (A)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.

Kat Moore (K)

Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands.

Sigrid Kolders (S)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands.

Hayley E Francies (HE)

Wellcome Sanger Institute, Hinxton CB10 1SA, United Kingdom.

M Can Gulersonmez (MC)

Department of Molecular Cancer Research, Center Molecular Medicine, Oncode Institute, University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Edwin C A Stigter (ECA)

Department of Molecular Cancer Research, Center Molecular Medicine, Oncode Institute, University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Boudewijn Burgering (B)

Department of Molecular Cancer Research, Center Molecular Medicine, Oncode Institute, University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Veerle Geurts (V)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands.

Ana Gracanin (A)

Hubrecht Organoid Technology, Utrecht 3584 CM, The Netherlands.

Gergana Bounova (G)

Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands.

Folkert H Morsink (FH)

Department of Pathology, University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Robert Vries (R)

Hubrecht Organoid Technology, Utrecht 3584 CM, The Netherlands.

Sylvia Boj (S)

Hubrecht Organoid Technology, Utrecht 3584 CM, The Netherlands.

Johan van Es (J)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands.

G Johan A Offerhaus (GJA)

Department of Pathology, University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Onno Kranenburg (O)

Utrecht Platform for Organoid Technology, Utrecht Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Mathew J Garnett (MJ)

Wellcome Sanger Institute, Hinxton CB10 1SA, United Kingdom.

Lodewyk Wessels (L)

Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands.

Edwin Cuppen (E)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands.
Hartwig Medical Foundation, 1098 XH Amsterdam, The Netherlands.
Center for Personalized Cancer Treatment,University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Lodewijk A A Brosens (LAA)

Department of Pathology, University Medical Center Utrecht, Utrecht 3584 CM, The Netherlands.

Hans Clevers (H)

Oncode Institute, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, 3584 CT Utrecht, The Netherlands.
Princess Maxima Center, Utrecht 3584 CS, The Netherlands.

Classifications MeSH