Culture of Methanogenic Archaea from Human Colostrum and Milk.
Animals
Body Mass Index
Breast Feeding
/ adverse effects
Chemoautotrophic Growth
/ genetics
Colostrum
/ microbiology
DNA, Archaeal
/ genetics
Euryarchaeota
/ genetics
Feces
/ microbiology
Female
Humans
Infant
Methanobrevibacter
/ genetics
Microbiota
/ genetics
Milk, Human
/ microbiology
Mothers
Pregnancy
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 12 2019
09 12 2019
Historique:
received:
28
01
2019
accepted:
05
11
2019
entrez:
11
12
2019
pubmed:
11
12
2019
medline:
11
11
2020
Statut:
epublish
Résumé
Archaeal sequences have been detected in human colostrum and milk, but no studies have determined whether living archaea are present in either of these fluids. Methanogenic archaea are neglected since they are not detected by usual molecular and culture methods. By using improved DNA detection protocols and microbial culture techniques associated with antioxidants previously developed in our center, we investigated the presence of methanogenic archaea using culture and specific Methanobrevibacter smithii and Methanobrevibacter oralis real-time PCR in human colostrum and milk. M. smithii was isolated from 3 colostrum and 5 milk (day 10) samples. M. oralis was isolated from 1 milk sample. For 2 strains, the genome was sequenced, and the rhizome was similar to that of strains previously isolated from the human mouth and gut. M. smithii was detected in the colostrum or milk of 5/13 (38%) and 37/127 (29%) mothers by culture and qPCR, respectively. The different distribution of maternal body mass index according to the detection of M. smithii suggested an association with maternal metabolic phenotype. M. oralis was not detected by molecular methods. Our results suggest that breastfeeding may contribute to the vertical transmission of these microorganisms and may be essential to seed the infant's microbiota with these neglected critical commensals from the first hour of life.
Identifiants
pubmed: 31819085
doi: 10.1038/s41598-019-54759-x
pii: 10.1038/s41598-019-54759-x
pmc: PMC6901439
doi:
Substances chimiques
DNA, Archaeal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18653Références
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