Sequential Immunization With Heterologous Viruses Does Not Result in Attrition of the B Cell Memory in Rainbow Trout.
Animals
Antibodies, Neutralizing
/ blood
Antibodies, Viral
/ blood
B-Lymphocytes
/ immunology
Birnaviridae Infections
/ prevention & control
Immunization
/ methods
Immunologic Memory
Infectious pancreatic necrosis virus
/ immunology
Novirhabdovirus
/ immunology
Oncorhynchus mykiss
/ blood
Rhabdoviridae Infections
/ prevention & control
Viral Vaccines
/ administration & dosage
B cell repertoire
RepSeq
antibodies
comparative immunology
fish immunology
heterologous immunization
public response
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2019
2019
Historique:
received:
13
08
2019
accepted:
31
10
2019
entrez:
12
12
2019
pubmed:
12
12
2019
medline:
18
11
2020
Statut:
epublish
Résumé
Long-term immunity is of great importance for protection against pathogens and has been extensively studied in mammals. Successive heterologous infections can affect the maintenance of immune memory, inducing attrition of T memory cells and diminishing B cell mediated protection. In fish, the basis of immune memory and the mechanisms of immunization to heterologous pathogens remain poorly understood. We sequentially immunized isogenic rainbow trout with two immunologically distinct viruses, VHSV and IPNV, either with one virus only or in combination, and analyzed the antibody responses and repertoires. Neutralizing antibodies and ELISPOT did not reveal an effect of heterologous immunization. Using a consensus read sequencing approach that incorporates unique barcodes to each cDNA molecule, we focused on the diversity expressed by selected responding VH/C combinations. We identified both public and private responses against VHSV and/or IPNV in all groups of fish. In fish immunized with two viruses, we registered no significant reduction in the persistence of the response toward the primary immunization. Similarly, the response to the second immunization was not affected by a prior vaccination to the other virus. Our data suggest that heterologous immunization does not enforce attrition of pre-existing antibody producing cells, which may impair the protection afforded by multiple successive vaccinations. These observations are potentially important to improve vaccination strategies practiced in aquaculture.
Identifiants
pubmed: 31824488
doi: 10.3389/fimmu.2019.02687
pmc: PMC6882293
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Viral Vaccines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2687Informations de copyright
Copyright © 2019 Navelsaker, Magadan, Jouneau, Quillet, Olesen, Munang'andu, Boudinot and Evensen.
Références
Clin Exp Immunol. 2018 Nov;194(2):192-204
pubmed: 30076783
Immunity. 1999 Dec;11(6):733-42
pubmed: 10626895
Vaccine. 2012 Jun 6;30(26):3918-28
pubmed: 22504037
Dev Comp Immunol. 2010 Dec;34(12):1291-9
pubmed: 20705088
Vet Res. 2013 Feb 11;44:7
pubmed: 23398909
J Immunol. 2004 Mar 1;172(5):3139-50
pubmed: 14978120
EMBO J. 1992 Dec;11(12):4337-47
pubmed: 1425571
Dev Comp Immunol. 2011 Dec;35(12):1273-81
pubmed: 21477614
Nat Rev Immunol. 2006 Oct;6(10):741-50
pubmed: 16977339
PLoS One. 2013;8(1):e54263
pubmed: 23349841
Front Immunol. 2019 Oct 02;10:2324
pubmed: 31632403
Immunity. 2004 Jan;20(1):5-16
pubmed: 14738760
PLoS Pathog. 2014 Jan;10(1):e1003843
pubmed: 24391499
J Immunol. 2006 Apr 1;176(7):4284-95
pubmed: 16547266
Methods Mol Biol. 2012;882:569-604
pubmed: 22665256
PLoS Pathog. 2008 May 30;4(5):e1000078
pubmed: 18516300
J Fish Dis. 2017 Dec;40(12):1775-1781
pubmed: 28493514
Front Immunol. 2018 Sep 27;9:2115
pubmed: 30319606
Fish Shellfish Immunol. 2019 Feb;85:3-8
pubmed: 29410093
Immunol Rev. 2010 May;235(1):244-66
pubmed: 20536568
Fish Shellfish Immunol. 2007 May;22(5):510-9
pubmed: 17085058
Immunity. 2009 Dec 18;31(6):859-71
pubmed: 20064446
PLoS One. 2013;8(4):e60175
pubmed: 23565199
PLoS Pathog. 2013 Jan;9(1):e1003098
pubmed: 23326228
J Immunol. 2001 Dec 1;167(11):6202-9
pubmed: 11714781
N Engl J Med. 2007 Nov 8;357(19):1903-15
pubmed: 17989383
Trends Immunol. 2010 Apr;31(4):144-53
pubmed: 20181529
PLoS Biol. 2018 Aug 10;16(8):e2006601
pubmed: 30096134
J Immunol. 2005 Jun 1;174(11):6608-16
pubmed: 15905499
Virology. 2004 Apr 25;322(1):31-40
pubmed: 15063114
J Immunol. 2004 Dec 15;173(12):7317-23
pubmed: 15585855
J Infect Dis. 2006 Jan 1;193(1):49-53
pubmed: 16323131
J Immunol. 2010 Jan 15;184(2):844-50
pubmed: 20018610
Sci Immunol. 2016 Oct 21;1(4):
pubmed: 27872905
J Virol. 2001 Jul;75(13):5965-76
pubmed: 11390598
Nature. 2009 Jan 8;457(7226):196-9
pubmed: 19005468