Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
11 12 2019
11 12 2019
Historique:
received:
27
11
2018
accepted:
11
11
2019
entrez:
13
12
2019
pubmed:
13
12
2019
medline:
12
3
2020
Statut:
epublish
Résumé
Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.
Identifiants
pubmed: 31827102
doi: 10.1038/s41467-019-13605-4
pii: 10.1038/s41467-019-13605-4
pmc: PMC6906306
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5658Références
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