Single cell transcriptional signatures of the human placenta in term and preterm parturition.
chorioamniotic membranes
decidua
genetics
genomics
human
human biology
labor
maternal-fetal interface
medicine
placenta
scRNA-seq
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
12 12 2019
12 12 2019
Historique:
received:
19
09
2019
accepted:
12
12
2019
pubmed:
13
12
2019
medline:
27
5
2020
entrez:
13
12
2019
Statut:
epublish
Résumé
More than 135 million births occur each year; yet, the molecular underpinnings of human parturition in gestational tissues, and in particular the placenta, are still poorly understood. The placenta is a complex heterogeneous organ including cells of both maternal and fetal origin, and insults that disrupt the maternal-fetal dialogue could result in adverse pregnancy outcomes such as preterm birth. There is limited knowledge of the cell type composition and transcriptional activity of the placenta and its compartments during physiologic and pathologic parturition. To fill this knowledge gap, we used scRNA-seq to profile the placental villous tree, basal plate, and chorioamniotic membranes of women with or without labor at term and those with preterm labor. Significant differences in cell type composition and transcriptional profiles were found among placental compartments and across study groups. For the first time, two cell types were identified: 1) lymphatic endothelial decidual cells in the chorioamniotic membranes, and 2) non-proliferative interstitial cytotrophoblasts in the placental villi. Maternal macrophages from the chorioamniotic membranes displayed the largest differences in gene expression (e.g.
Identifiants
pubmed: 31829938
doi: 10.7554/eLife.52004
pii: 52004
pmc: PMC6949028
doi:
pii:
Banques de données
dbGaP
['phs001886.v1.p1']
GEO
['GSE114037', 'GSE96083']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NICHD NIH HHS
ID : HHSN275201300006C
Pays : United States
Organisme : Wayne State University
ID : Perinatal Research Initiative
Pays : International
Organisme : NICHD NIH HHS
ID : HHSN275201300006C
Pays : United States
Déclaration de conflit d'intérêts
RP, RR, AT, ES, YX, VG, YL, FL, SH, NG No competing interests declared
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