The reduction in sexual behavior of adult female rats exposed to immune stress in the neonatal period is associated with reduced hypothalamic progesterone receptor expression.
Age Factors
Animals
Animals, Newborn
Down-Regulation
/ drug effects
Drug Administration Schedule
Female
Gene Expression
/ drug effects
Hypothalamus
/ metabolism
Immune System
/ drug effects
Lipopolysaccharides
/ administration & dosage
Male
Pregnancy
Rats
Rats, Sprague-Dawley
Receptors, Progesterone
/ genetics
Sexual Behavior, Animal
/ drug effects
Stress, Physiological
/ drug effects
Time Factors
Immune stress
Lipopolysaccharide
Postnatal
Progesterone receptor
Sexual behavior
Sexual maturation
Journal
General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735
Informations de publication
Date de publication:
01 03 2020
01 03 2020
Historique:
received:
28
08
2019
revised:
04
12
2019
accepted:
07
12
2019
pubmed:
13
12
2019
medline:
24
6
2020
entrez:
13
12
2019
Statut:
ppublish
Résumé
We examined the mechanism by which neonatal immune stress reduces the sexual behavior of female rats in adulthood. Neonatal female rats were randomly divided into 3 groups: control (n = 11), postnatal day 10 lipopolysaccharide (PND10LPS) (n = 23), and PND25LPS (n = 11) groups, which received intraperitoneal injections of LPS (100 μg/kg) or saline on PND10 and 25. Daily inspections of the vaginal opening (VO) were performed from PND27 to PND37. Thereafter, the frequency of estrus was assessed for 15 days. Female rats (at 11-12 weeks of age) were placed in a cage with male rats, and their sexual behavior was monitored for 30 min. The hypothalamic mRNA expression levels of factors related to sexual behavior were examined via real-time PCR. VO occurred later and the frequency of estrus was lower in the PND10LPS group compared to the control group. The number of lordosis behaviors and the total number of mounts performed by male partners were lower in the PND10LPS and PND25LPS groups than in the control group. Acceptability: The lordosis quotient and lordosis rating were lower in the PND10LPS group than in the control group. Proceptive behavior: the number of ear wiggling events was lower in the PND10LPS group than in the other groups, and the number of hops/darts was lower in the PND10LPS group than in the control group. The hypothalamic mRNA expression level of progesterone receptors (PR)A + B was lower in the PND10LPS group than in the control group, and the hypothalamic PRB mRNA expression level was lower in the PND10LPS and PND25LPS groups than in the control group. Neonatal immune stress impeded sexual behavior and hypothalamic PR mRNA expression in female rats. Decreased progesterone activity in the hypothalamus might explain the reduction in sexual behavior seen in these rats.
Identifiants
pubmed: 31830472
pii: S0016-6480(19)30460-5
doi: 10.1016/j.ygcen.2019.113360
pii:
doi:
Substances chimiques
Lipopolysaccharides
0
Receptors, Progesterone
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113360Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.