Functional recombinant apolipoprotein A5 that is stable at high concentrations at physiological pH.
dyslipidemias
lipid and lipoprotein metabolism
lipoproteins
triglycerides
Journal
Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
30
04
2019
revised:
25
11
2019
pubmed:
14
12
2019
medline:
9
2
2021
entrez:
14
12
2019
Statut:
ppublish
Résumé
APOA5 is a low-abundance exchangeable apolipoprotein that plays critical roles in human triglyceride (TG) metabolism. Indeed, aberrations in the plasma concentration or structure of APOA5 are linked to hypertriglyceridemia, hyperchylomicronemia, myocardial infarction risk, obesity, and coronary artery disease. While it has been successfully produced at low yield in bacteria, the resulting protein had limitations for structure-function studies due to its low solubility under physiological buffer conditions. We hypothesized that the yield and solubility of recombinant APOA5 could be increased by:
Identifiants
pubmed: 31831525
pii: S0022-2275(20)43540-0
doi: 10.1194/jlr.D119000103
pmc: PMC6997596
doi:
Substances chimiques
APOA5 protein, human
0
Apolipoprotein A-V
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
244-251Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM134923
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL125204
Pays : United States
Informations de copyright
Copyright © 2020 Castleberry et al.
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