Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Animals
Brain
/ immunology
Brain Injuries
/ immunology
Calcium
Cell Communication
/ immunology
HEK293 Cells
Humans
Intercellular Junctions
/ immunology
Mice
Microglia
/ immunology
Mitochondria
/ immunology
Neurons
/ immunology
Receptors, Purinergic P2Y12
/ physiology
Shab Potassium Channels
/ genetics
Signal Transduction
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
31 01 2020
31 01 2020
Historique:
received:
12
04
2019
revised:
14
10
2019
accepted:
03
12
2019
pubmed:
14
12
2019
medline:
16
4
2020
entrez:
14
12
2019
Statut:
ppublish
Résumé
Microglia are the main immune cells in the brain and have roles in brain homeostasis and neurological diseases. Mechanisms underlying microglia-neuron communication remain elusive. Here, we identified an interaction site between neuronal cell bodies and microglial processes in mouse and human brain. Somatic microglia-neuron junctions have a specialized nanoarchitecture optimized for purinergic signaling. Activity of neuronal mitochondria was linked with microglial junction formation, which was induced rapidly in response to neuronal activation and blocked by inhibition of P2Y12 receptors. Brain injury-induced changes at somatic junctions triggered P2Y12 receptor-dependent microglial neuroprotection, regulating neuronal calcium load and functional connectivity. Thus, microglial processes at these junctions could potentially monitor and protect neuronal functions.
Identifiants
pubmed: 31831638
pii: science.aax6752
doi: 10.1126/science.aax6752
doi:
Substances chimiques
Receptors, Purinergic P2Y12
0
Shab Potassium Channels
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
528-537Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM084136
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM109888
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.