A microfluidic mammary gland coculture model using parallel 3D lumens for studying epithelial-endothelial migration in breast cancer.
Journal
Biomicrofluidics
ISSN: 1932-1058
Titre abrégé: Biomicrofluidics
Pays: United States
ID NLM: 101293825
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
13
08
2019
accepted:
06
11
2019
entrez:
14
12
2019
pubmed:
14
12
2019
medline:
14
12
2019
Statut:
epublish
Résumé
In breast cancer development, crosstalk between mammary epithelial cells and neighboring vascular endothelial cells is critical to understanding tumor progression and metastasis, but the mechanisms of this dynamic interplay are not fully understood. Current cell culture platforms do not accurately recapitulate the 3D luminal architecture of mammary gland elements. Here, we present the development of an accessible and scalable microfluidic coculture system that incorporates two parallel 3D luminal structures that mimic vascular endothelial and mammary epithelial cell layers, respectively. This parallel 3D lumen configuration allows investigation of endothelial-epithelial crosstalk and its effects of the comigration of endothelial and epithelial cells into microscale migration ports located between the parallel lumens. We describe the development and application of our platform, demonstrate generation of 3D luminal cell layers for endothelial cells and three different breast cancer cell lines, and quantify their migration profiles based on number of migrated cells, area coverage by migrated cells, and distance traveled by individual migrating cells into the migration ports. Our system enables analysis at the single-cell level, allows simultaneous monitoring of endothelial and epithelial cell migration within a 3D extracellular matrix, and has potential for applications in basic research on cellular crosstalk as well as drug development.
Identifiants
pubmed: 31832120
doi: 10.1063/1.5123912
pii: 1.5123912
pmc: PMC6894982
doi:
Types de publication
Journal Article
Langues
eng
Pagination
064122Informations de copyright
Copyright © 2019 Author(s).
Références
Lab Chip. 2015 Jan 7;15(1):301-10
pubmed: 25370780
Oncotarget. 2014 Dec 15;5(23):12383-97
pubmed: 25474037
Biomed Microdevices. 2016 Aug;18(4):70
pubmed: 27432323
Integr Biol (Camb). 2013 Sep;5(9):1096-109
pubmed: 23799587
FASEB J. 2014 Nov;28(11):4583-90
pubmed: 25077562
J Cell Biochem. 2012 Apr;113(4):1142-51
pubmed: 22095586
Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):13515-20
pubmed: 22869695
Biomech Model Mechanobiol. 2019 Jun;18(3):717-731
pubmed: 30604299
Biomicrofluidics. 2014 Sep 04;8(5):054102
pubmed: 25332739
Integr Biol (Camb). 2014 Jun;6(6):627-635
pubmed: 24791272
Lab Chip. 2007 Nov;7(11):1475-8
pubmed: 17960274
J Biomed Mater Res A. 2013 Oct;101(10):2948-56
pubmed: 23559519
BMC Cancer. 2015 Jan 21;15:12
pubmed: 25605670
Integr Biol (Camb). 2019 Apr 1;11(4):119-129
pubmed: 31125041
Integr Biol (Camb). 2014 May;6(5):555-63
pubmed: 24676392
Eur J Cancer. 2009 Jul;45(11):1924-34
pubmed: 19406633
Clin Anat. 2013 Jan;26(1):29-48
pubmed: 22997014
Cancer Res. 2014 May 15;74(10):2869-81
pubmed: 24686166
Lab Chip. 2010 Jan 21;10(2):143-60
pubmed: 20066241
Nat Rev Cancer. 2013 Dec;13(12):858-70
pubmed: 24263189
Cancer Immunol Res. 2017 Mar;5(3):234-247
pubmed: 28159748
Br J Cancer. 2008 Dec 16;99(12):1961-6
pubmed: 18941463
Nat Rev Cancer. 2017 Aug;17(8):457-474
pubmed: 28706266
Clin Chem. 2003 Jan;49(1):32-40
pubmed: 12507958
J Cell Sci. 2012 Jul 1;125(Pt 13):3015-24
pubmed: 22797912
J Biomed Mater Res. 2002 Jan;59(1):160-5
pubmed: 11745549
Lab Chip. 2015 Nov 7;15(21):4114-27
pubmed: 26334370
Nat Rev Cancer. 2007 Sep;7(9):659-72
pubmed: 17721431
J Cell Sci. 2003 Jun 15;116(Pt 12):2377-88
pubmed: 12766184
Integr Biol (Camb). 2015 Jun;7(6):643-54
pubmed: 25998180
Annu Rev Biomed Eng. 2001;3:335-73
pubmed: 11447067
Nat Commun. 2017 Aug 15;8(1):262
pubmed: 28811479
Biosens Bioelectron. 2020 Jan 1;147:111757
pubmed: 31654819
J Natl Cancer Inst. 1982 Aug;69(2):371-80
pubmed: 6287076
Science. 2006 Oct 13;314(5797):298-300
pubmed: 17038622
Annu Rev Cell Dev Biol. 2006;22:287-309
pubmed: 16824016
Physiology (Bethesda). 2017 Jul;32(4):266-277
pubmed: 28615311
Adv Drug Deliv Rev. 2014 Apr;69-70:42-51
pubmed: 24412474
Blood. 2006 Mar 1;107(5):1761-7
pubmed: 16269611
Breast Cancer Res. 2008;10(2):R26
pubmed: 18371194
Lab Chip. 2017 Dec 5;17(24):4171-4185
pubmed: 28971203
Cancer Cell. 2015 Jun 8;27(6):755-68
pubmed: 26058075
EBioMedicine. 2019 Apr;42:408-419
pubmed: 30902740
Lab Chip. 2017 Aug 22;17(17):2960-2968
pubmed: 28740980
Br J Cancer. 2009 Aug 18;101(4):666-72
pubmed: 19623180
PLoS One. 2011;6(9):e25661
pubmed: 21984937
Oncogene. 2016 Feb 11;35(6):748-60
pubmed: 25961925
Biomaterials. 2013 Feb;34(5):1471-7
pubmed: 23191982
Annu Rev Biomed Eng. 2012;14:205-30
pubmed: 22540941
Anal Chem. 2012 Feb 21;84(4):2088-93
pubmed: 22263607
Sci Rep. 2015 Dec 03;5:17768
pubmed: 26631692
Biomicrofluidics. 2017 Oct 10;11(5):054111
pubmed: 29085522
Anal Chem. 2015 Mar 17;87(6):3239-46
pubmed: 25719435
Integr Biol (Camb). 2012 Aug;4(8):857-62
pubmed: 22673771
Oncogene. 2004 Feb 12;23(6):1275-82
pubmed: 14676843
Blood. 2012 Mar 8;119(10):e76-85
pubmed: 22262772
Curr Opin Cell Biol. 2005 Oct;17(5):524-32
pubmed: 16112853
Biotechnol Bioeng. 2008 Aug 15;100(6):1205-13
pubmed: 18553401
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6712-7
pubmed: 23569284
J Lab Autom. 2013 Dec;18(6):427-36
pubmed: 23832929
Chem Soc Rev. 2010 Mar;39(3):1036-48
pubmed: 20179823
ACS Biomater Sci Eng. 2017 Nov 13;3(11):2999-3006
pubmed: 33418720
Oncogene. 2017 May 11;36(19):2680-2692
pubmed: 27893712
Cell Mol Bioeng. 2014 Sep;7(3):460-472
pubmed: 25484989
Proc Natl Acad Sci U S A. 2011 Feb 22;108(8):3264-9
pubmed: 21300877
Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15342-7
pubmed: 21876168