Biomechanical and Morphologic Effects of Collagen Cross-Linking in Human Tarsus.

cross-linking floppy eyelids tarsus

Journal

Translational vision science & technology
ISSN: 2164-2591
Titre abrégé: Transl Vis Sci Technol
Pays: United States
ID NLM: 101595919

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 21 02 2019
accepted: 03 07 2019
entrez: 14 12 2019
pubmed: 14 12 2019
medline: 14 12 2019
Statut: epublish

Résumé

To investigate the feasibility of increasing the stiffness of human tarsal tissue following treatment with riboflavin and ultraviolet A (UVA) to induce cross-linking of collagen fibers. In this case control study, 18 right and left upper eyelids were excised en bloc from 18 fresh-frozen cadavers. One side served as the control while the samples from the opposite side were cross-linked. Four 2 × 6-mm vertical strips of central tarsus were cut from the superior to inferior border of each tarsal plate. Sample tissue was irradiated with UVA at 6 mW/cm Mean Young's modulus in the linear region for controls was 28 ± 9 MPa and was much higher at 138 ± 8 MPa for cross-linked samples ( The findings of this study suggest that collagen cross-linking is a viable and effective modality for increasing the stiffness of human tarsal plates. This work provides proof that collagen cross-linking produces stiffening of the human tarsal plate and may be used in disorders that cause eyelid laxity.

Identifiants

pubmed: 31832279
doi: 10.1167/tvst.8.6.25
pii: TVST-19-1458
pmc: PMC6900964
doi:

Types de publication

Journal Article

Langues

eng

Pagination

25

Subventions

Organisme : NEI NIH HHS
ID : P30 EY000331
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY008313
Pays : United States

Informations de copyright

Copyright 2019 The Authors.

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Auteurs

Shoaib Ugradar (S)

Stein Eye Institute, University of California, Los Angeles, CA, USA.

Alan Le (A)

Bioengineering Interdepartmental Program, University of California, Los Angeles, CA, USA.

Michael Lesgart (M)

Stein Eye Institute, University of California, Los Angeles, CA, USA.

Robert A Goldberg (RA)

Stein Eye Institute, University of California, Los Angeles, CA, USA.

Daniel Rootman (D)

Stein Eye Institute, University of California, Los Angeles, CA, USA.
Doheny Eye Institute, University of California, Los Angeles, CA, USA.
Neuroscience Interdepartmental Program, University of California, Los Angeles, CA, USA.

Joseph L Demer (JL)

Stein Eye Institute, University of California, Los Angeles, CA, USA.
Bioengineering Interdepartmental Program, University of California, Los Angeles, CA, USA.
Department of Neurology, University of California, Los Angeles, CA, USA.
Neuroscience Interdepartmental Program, University of California, Los Angeles, CA, USA.

Classifications MeSH