Liquid-liquid phase separation of the Golgi matrix protein GM130.
Golgi matrix
Golgin
coiled-coil
liquid
liquid phase separation
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
08
11
2019
revised:
26
11
2019
accepted:
02
12
2019
pubmed:
14
12
2019
medline:
10
4
2021
entrez:
14
12
2019
Statut:
ppublish
Résumé
Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50-400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as vesicle tethers, Golgins may also possess the capacity to phase separate and, thereby, contribute to the internal organization of the Golgi. GM130 is the most abundant Golgin at the cis Golgi. Remarkably, overexpressed GM130 forms liquid droplets in cells analogous to those described for numerous intrinsically disordered proteins with low complexity sequences, even though GM130 is neither low in complexity nor intrinsically disordered. Virtually pure recombinant GM130 also phase-separates into dynamic, liquid-like droplets in close to physiological buffers and at concentrations similar to its estimated local concentration at the cis Golgi.
Identifiants
pubmed: 31833055
doi: 10.1002/1873-3468.13715
pmc: PMC7160038
mid: NIHMS1063365
doi:
Substances chimiques
Autoantigens
0
Golgin subfamily A member 2
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1132-1144Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK034989
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118084
Pays : United States
Organisme : NIH HHS
ID : S10 OD020142
Pays : United States
Informations de copyright
© 2019 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Références
Mol Cell. 2015 Mar 5;57(5):936-947
pubmed: 25747659
Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7189-94
pubmed: 26015579
Cell. 1989 Mar 10;56(5):801-13
pubmed: 2647301
Cell. 2015 Jul 16;162(2):287-299
pubmed: 26165940
EMBO J. 2018 Apr 3;37(7):
pubmed: 29472250
Nat Rev Mol Cell Biol. 2017 May;18(5):285-298
pubmed: 28225081
Cell. 1993 Jun 18;73(6):1079-90
pubmed: 8513494
Plant Physiol. 2008 Aug;147(4):1454-68
pubmed: 18678738
Mol Biol Cell. 1997 Jun;8(6):1073-87
pubmed: 9201717
Nat Cell Biol. 2006 Mar;8(3):238-48
pubmed: 16489344
Cell. 2015 Sep 24;163(1):123-33
pubmed: 26406374
Science. 1994 Feb 11;263(5148):802-5
pubmed: 8303295
J Mol Biol. 2018 Nov 2;430(23):4806-4820
pubmed: 29944854
Annu Rev Cell Dev Biol. 2002;18:379-420
pubmed: 12142281
Nature. 2012 Mar 07;483(7389):336-40
pubmed: 22398450
Elife. 2018 Nov 30;7:
pubmed: 30499774
Elife. 2016 Jun 09;5:
pubmed: 27278775
FEBS Lett. 2019 Oct;593(19):2701-2705
pubmed: 31562650
Science. 2009 Jun 26;324(5935):1729-32
pubmed: 19460965
Cell. 1997 May 2;89(3):445-55
pubmed: 9150144
Elife. 2014 Oct 16;3:
pubmed: 25321392
Cell. 2012 May 11;149(4):753-67
pubmed: 22579281
Annu Rev Cell Dev Biol. 2014;30:39-58
pubmed: 25288112
Trends Cell Biol. 2016 Jun;26(6):399-408
pubmed: 26972448
J Cell Biol. 1995 Dec;131(6 Pt 2):1715-26
pubmed: 8557739
Biomolecules. 2019 Feb 19;9(2):
pubmed: 30791483
Cell. 2007 Aug 10;130(3):512-23
pubmed: 17693259
Science. 1975 Aug 1;189(4200):347-58
pubmed: 1096303
Mol Biol Cell. 2019 Jan 15;30(2):173-180
pubmed: 30640588
PLoS One. 2016 Jul 07;11(7):e0158457
pubmed: 27387979
Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4334-9
pubmed: 21368180
J Cell Biol. 2001 Oct 15;155(2):181-5
pubmed: 11604416
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
FEBS J. 2015 Jun;282(11):2232-44
pubmed: 25787021
Cell. 2016 Jun 16;165(7):1686-1697
pubmed: 27212236
PLoS One. 2011;6(8):e23519
pubmed: 21901122