Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program.
immunology
microglia
neurodegeneration
single-cell RNA-seq
systems biology
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
12 12 2019
12 12 2019
Historique:
received:
12
03
2019
revised:
30
07
2019
accepted:
06
11
2019
entrez:
14
12
2019
pubmed:
14
12
2019
medline:
6
6
2020
Statut:
ppublish
Résumé
Microglia, the brain-resident immune cells, are critically involved in many physiological and pathological brain processes, including neurodegeneration. Here we characterize microglia morphology and transcriptional programs across ten species spanning more than 450 million years of evolution. We find that microglia express a conserved core gene program of orthologous genes from rodents to humans, including ligands and receptors associated with interactions between glia and neurons. In most species, microglia show a single dominant transcriptional state, whereas human microglia display significant heterogeneity. In addition, we observed notable differences in several gene modules of rodents compared with primate microglia, including complement, phagocytic, and susceptibility genes to neurodegeneration, such as Alzheimer's and Parkinson's disease. Our study provides an essential resource of conserved and divergent microglia pathways across evolution, with important implications for future development of microglia-based therapies in humans.
Identifiants
pubmed: 31835035
pii: S0092-8674(19)31231-0
doi: 10.1016/j.cell.2019.11.010
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1609-1622.e16Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/D/20320000
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.