Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation.
Amino Acids
/ metabolism
Animals
Bile
/ metabolism
Blastocyst
/ metabolism
Chromatography, High Pressure Liquid
Embryonic Development
/ physiology
Energy Metabolism
Female
Gas Chromatography-Mass Spectrometry
Gestational Age
Male
Metabolome
/ physiology
Metabolomics
/ methods
Pregnancy
Pregnancy, Animal
/ metabolism
Proteins
/ metabolism
Purines
/ metabolism
Swine
/ embryology
Uterus
/ metabolism
blastocyst elongation
metabolome
porcine
uterine environment
Journal
Molecular reproduction and development
ISSN: 1098-2795
Titre abrégé: Mol Reprod Dev
Pays: United States
ID NLM: 8903333
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
24
09
2019
accepted:
02
12
2019
pubmed:
17
12
2019
medline:
9
2
2021
entrez:
17
12
2019
Statut:
ppublish
Résumé
The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross-bred gilts (n = 38) were collected immediately following harvest and flushed with Roswell Park Memorial Institute-1640 medium. Conceptus morphologies were assessed from each pregnancy and corresponding uterine flushings were assigned to one of five treatment groups based on these morphologies: (a) uniform spherical (n = 8); (b) heterogeneous spherical and ovoid (n = 8); (c) uniform ovoid (n = 8); (d) heterogeneous ovoid and tubular (n = 8); and (e) uniform tubular (n = 6). Uterine flushings from these pregnancies were submitted for nontargeted profiling by gas chromatography-mass spectrometry (GC-MS) and ultra performance liquid chromatography (UPLC)-MS techniques. Unsupervised multivariate principal component analysis (PCA) was performed using pcaMethods and univariate analysis of variance was performed in R with false discovery rate (FDR) adjustment. PCA analysis of the GC-MS and UPLC-MS data identified 153 and 104 metabolites, respectively. After FDR adjustment of the GC-MS and UPLC-MS data, 38 and 59 metabolites, respectively, differed (p < .05) in uterine flushings from pregnancies across the five conceptus stages. Some metabolites were greater (p < .05) in abundance for uterine flushings containing earlier stage conceptuses (i.e., spherical), such as uric acid, tryptophan, and tyrosine. In contrast, some metabolites were greater (p < .05) in abundance for uterine flushings containing later stage conceptuses (i.e., tubular), such as creatinine, serine, and urea. These data illustrate several putative metabolites that change within the uterine milieu during early porcine blastocyst elongation.
Identifiants
pubmed: 31840336
doi: 10.1002/mrd.23306
pmc: PMC7003770
doi:
Substances chimiques
Amino Acids
0
Proteins
0
Purines
0
purine
W60KTZ3IZY
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
174-190Informations de copyright
© 2019 The Authors. Molecular Reproduction and Development Published by Wiley Periodicals, Inc.
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