Red, Orange, Green: Light- and Temperature-Dependent Color Tuning in a Cyanobacteriochrome.


Journal

Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623

Informations de publication

Date de publication:
04 02 2020
Historique:
pubmed: 17 12 2019
medline: 22 8 2020
entrez: 17 12 2019
Statut: ppublish

Résumé

Cyanobacteriochromes (CBCRs) are photoreceptor proteins that photoconvert between two parent states and thereby regulate various biological processes. An intriguing property is their variable ultraviolet-visible (UV-vis) absorption that covers the entire spectral range from the far-red to the near-UV region and thus makes CBCRs promising candidates for optogenetic applications. Here, we have studied Slr1393, a CBCR that photoswitches between red- and green-absorbing states (Pr and Pg, respectively). Using UV-vis absorption, fluorescence, and resonance Raman (RR) spectroscopy, a further orange-absorbing state O

Identifiants

pubmed: 31840994
doi: 10.1021/acs.biochem.9b00931
doi:

Substances chimiques

Bacterial Proteins 0
Photoreceptors, Microbial 0
Phycobilins 0
Pigments, Biological 0
Phycocyanin 11016-15-2
Phytochrome 11121-56-5
phycocyanobilin 36NUT04V2K

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

509-519

Auteurs

David Buhrke (D)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Giovanni Battocchio (G)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Svea Wilkening (S)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Matthew Blain-Hartung (M)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Tobias Baumann (T)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Franz-Josef Schmitt (FJ)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Thomas Friedrich (T)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Maria-Andrea Mroginski (MA)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

Peter Hildebrandt (P)

Technische Universität Berlin , Faculty II-Mathematics and Natural Sciences, Institute of Chemistry , Sekr. PC14, Straße des 17. Juni 135 , D-10623 Berlin , Germany.

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Classifications MeSH