FUNCTION OF EXTERNAL RESPIRATION AND MARKERS OF OXIDATIVE STRESS IN CHILDREN-RESIDENTS OF RADIOACTIVELY CONTAMINATED TERRITORIES.

to evaluate the influence of processes of lipid peroxidation, and antioxidant protection on the function of external respiration in children-residents of radioactive contaminated territories. There were examined children of school age, inhabitants of radioactive contaminated ter- ritories (RCT) without respiratory and pulmonary pathology, and patients with bronchial asthma (BA). Examination of the ventilation lung function was performed by the method of pneumotachography according to the analysis of the «flow-volume» loop. End products of lipid peroxidation (LPO), reacting with thiobarbituric acid (malondialde- hyde), enzymes-antioxidants - catalase, superoxide dismutase, glutathione peroxidase, glutathione transferase were studied to determine the signs of oxidative stress. Examinations of ventilation lung function in children-residents of RCT without respiratory and pulmonary pathology showed no significant deviations of its parameters beyond the limits of physiological fluctuations; a decrease in bronchial patency at different levels of the bronchial tree was established in children with BA. An increased incidence of bronchial hyperreactivity was noted in both the patients with BA and those who did not have bronchopulmonary pathology. The absence of an increase in the content of LPO products (malondialdehyde) in blood serum of children-residents of RCT with increased activity of catalase and group of glutathione enzymes indi- cates the sufficiency of compensatory possibilities of antioxidant protection. Studies of correlation between the function of external respiration and markers of oxidative stress determined that the indices of bronchial patency directly correlate with the activity of enzymes-antioxidants of the glutathione group and inversely - with the content of the LPO products in children-residents of RCT. The frequency of bronchospasm inversely correlated with the activity of glutathione group antioxidants. There are inverse correla- tion of 137Cs content in the body with the activity of glutathione transferase and glutathione peroxidase. Meta: otsinka vplyvu protsesiv perekysnogo okyslennia lipidiv ta antyoksydantnogo zakhystu na funktsiiu zovnish- n'ogo dykhannia u ditey̆ – meshkantsiv radioaktyvno zabrudnenykh terytoriy̆.Materialy ta metody. Obstezheni dity shkil'nogo viku – meshkantsi radioaktyvno zabrudnenykh terytoriy̆ (RZT) bez patologiï bronkhiv i legeniv ta khvori na bronkhial'nu astmu (BA). Doslidzhennia ventyliatsiy̆noï funktsiï legeniv pro- vodyly metodom pnevmotakhografiï za danymy analizu petli «potik–ob’iem». Dlia vyiavlennia oznak oksydatyvnogo stresu vyznachaly vmist u syrovattsi krovi kintsevykh produktiv perekysnogo okyslennia lipidiv, shcho reaguiut' z tiobarbiturovoiu kyslotoiu (malonovogo dial'degidu), ta aktyvnist' fermentiv-antyoksydantiv – katalazy, su- peroksyddysmutazy, glutationperoksydazy, glutationtransferazy.Rezul'taty. Doslidzhennia ventyliatsiy̆noï funktsiï legeniv u ditey̆ meshkantsiv RZT, iaki ne maly patologiï bronkhiv i legeniv, ne vyiavylo suttievykh vidkhylen' ïï pokaznykiv za mezhi fiziologichnykh kolyvan'; u ditey̆, khvorykh na BA, vstanovleno znyzhennia bronkhial'noï prokhidnosti na riznykh rivniakh bronkhial'nogo dereva. Sposterigalasia pidvy- shchena chastota bronkhial'noï giperreaktyvnosti iak u khvorykh na BA, tak i tykh, khto ne mav bronkholegenevoï patologiï. Vidsutnist' pidvyshchennia vmistu produktiv perekysnogo okyslennia lipidiv (malonovogo dial'degidu) v syrovattsi krovi ditey̆ – meshkantsiv RZT pry pidvyshcheniy̆ aktyvnosti katalazy ta fermentiv grupy glutationu svidchyt' pro dostatnist' kompensatornykh mozhlyvostey̆ antyoksydantnogo zakhystu.Vysnovky. Doslidzhennia koreliatsiy̆nykh zv’iazkiv funktsiï zovnishn'ogo dykhannia i markeriv oksydatyvnogo stresu pokazalo, shcho u ditey̆ – meshkantsiv RZT pokaznyky bronkhial'noï prokhidnosti priamo koreliuiut' z aktyvnistiu fer- mentiv-antyoksydantiv grupy glutationu i oberneno – z vmistom produktiv POL. Chastota bronkhospazmu oberneno koreliuie z aktyvnistiu antyoksydantiv grupy glutationu. Maiut' mistse oberneni koreliatsiy̆ni zv’iazky vmistu 137Cs v tili z aktyvnistiu glutationtransferazy ta glutationperoksydazy.

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Ye. I. Stepanova, I. Ye.Kolpakov, S. M. Alokhina, V. Yu. Vdovenko, V. M. Zyhalo, O. S. Leonovych.