Steroid profile analysis by LC-HRMS in human seminal fluid.
LC-HRMS
Seminal fluid
Steroid profile
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554
Informations de publication
Date de publication:
01 Jan 2020
01 Jan 2020
Historique:
received:
08
07
2019
revised:
05
11
2019
accepted:
04
12
2019
pubmed:
17
12
2019
medline:
4
3
2020
entrez:
17
12
2019
Statut:
ppublish
Résumé
Steroids are essential hormones that play a crucial role in homeostasis of many biological processes including sexual development, spermatogenesis, sperm physiology and fertility. Although steroids have been largely studied in many biological matrices (such as urine and plasma), there is very limited information of the steroid content and their study as potential indicators of the quality of the seminal fluid. In this study, a LC-HRMS (liquid chromatography-high resolution mass spectrometry) strategy has been developed in order to obtain the extended steroid profile of human seminal fluid. A comparison between supported liquid extraction (SLE) and solid liquid extraction (SPE) was carried out and the chosen SPE method was further optimized to evidence the largest possible number of compounds. Steroids were automatically annotated by using DynaStI, a publicly available retention time prediction tool developed in our lab, to match the experimental data (i.e. accurate mass and t
Identifiants
pubmed: 31841978
pii: S1570-0232(19)31038-4
doi: 10.1016/j.jchromb.2019.121929
pii:
doi:
Substances chimiques
Steroids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121929Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.