Resident microbes of lactation rooms and daycares.
Built environment microbiome
Daycares
Lactation rooms
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
06
2019
accepted:
06
11
2019
entrez:
18
12
2019
pubmed:
18
12
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Dedicated lactation rooms are a modern development as mothers return to work while still providing breastmilk to their absent infants. This study describes the built environment microbiome of lactation rooms and daycares, and explores the influence of temperature and humidity on the microbiome of lactation rooms. Sterile swabs were used to collect samples from five different sites in lactation rooms at University of California, Davis and from five different sites in daycares located in Davis, California. DNA from the swabs was extracted and the V4 region of the 16S rRNA gene was sequenced using Illumina MiSeq. Temperature and relative humidity data were collected on a subset of the lactation rooms. Sampled lactation rooms could be either dedicated lactation rooms or could also serve other functions (e.g., combined lactation room and restroom lounge). The majority of sequence reads were identified as belonging to family Moraxellaceae, with 73% of all reads included in analysis identified as an unknown species of
Identifiants
pubmed: 31844574
doi: 10.7717/peerj.8168
pii: 8168
pmc: PMC6913265
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e8168Subventions
Organisme : NICHD NIH HHS
ID : F32 HD093185
Pays : United States
Informations de copyright
© 2019 Taft et al.
Déclaration de conflit d'intérêts
Zachery T. Lewis is now an employee of The Clorox Company.
Références
Cell Host Microbe. 2019 Feb 13;25(2):324-335.e4
pubmed: 30763539
Environ Microbiol. 2009 Apr;11(4):809-22
pubmed: 19040453
Pediatrics. 2014 Aug;134(2):e362-72
pubmed: 25002669
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
PeerJ. 2015 Sep 22;3:e1258
pubmed: 26417541
ISME J. 2012 Aug;6(8):1469-79
pubmed: 22278670
ISME J. 2012 Mar;6(3):610-8
pubmed: 22134646
PLoS One. 2011;6(11):e28132
pubmed: 22132229
mSystems. 2016 Apr 19;1(2):
pubmed: 27832216
Microbiome. 2015 Oct 13;3:49
pubmed: 26459172
Matern Child Health J. 2014 Jan;18(1):286-295
pubmed: 23584928
J Clin Microbiol. 1997 Nov;35(11):2819-25
pubmed: 9350741
Breastfeed Rev. 2006 Nov;14(3):5-9
pubmed: 17190014
Bioinformatics. 2012 Aug 15;28(16):2106-13
pubmed: 22711789
N Engl J Med. 2011 Feb 24;364(8):701-9
pubmed: 21345099
PLoS Pathog. 2019 Feb 21;15(2):e1007563
pubmed: 30789972
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Proc Natl Acad Sci U S A. 2012 May 22;109(21):8334-9
pubmed: 22566627
Appl Environ Microbiol. 2014 Nov;80(21):6760-70
pubmed: 25172855
IUBMB Life. 2011 Dec;63(12):1048-54
pubmed: 22006724
mSystems. 2016 Apr 19;1(2):
pubmed: 27822521
Mol Biol Evol. 2009 Jul;26(7):1641-50
pubmed: 19377059
Nature. 2018 Mar 8;555(7695):210-215
pubmed: 29489753