Brain tumors
Copper transporters
Cu-ATSM
Hypoxia
PET radiotracers
Journal
EJNMMI research
ISSN: 2191-219X
Titre abrégé: EJNMMI Res
Pays: Germany
ID NLM: 101560946
Informations de publication
Date de publication:
19 Dec 2019
19 Dec 2019
Historique:
received:
19
09
2019
accepted:
10
12
2019
entrez:
21
12
2019
pubmed:
21
12
2019
medline:
21
12
2019
Statut:
epublish
Résumé
Diacetyl-bis(N4-methylthiosemicarbazone), labeled with 64Cu ( μPET was performed 14 days ( In vivo results showed that Cu-ASTM and Cu-Cl2 accumulated in hypoxic areas of the tumors. Cu-ATSM also stained, to a lesser extent, non-hypoxic regions, such as regions of astrogliosis, with high expression of copper transporters and in particular DMT-1 and CTR1, and also characterized by the expression of elevated astrogliosis. In vitro results show that 64Cu-ATSM showed an increase in the uptake only in severe hypoxia at 0.5 and 0.2% of oxygen while for In the present study, we show that Cu-complexes undoubtedly accumulate in hypoxic areas of the tumors. This uptake may be the reflection of a direct dependency to a redox metabolism and also a reflection of hypoxic-induced overexpression of transporters. We also show that Cu-ATSM also stained non-hypoxic regions such as astrogliosis.
Sections du résumé
BACKGROUND
BACKGROUND
Diacetyl-bis(N4-methylthiosemicarbazone), labeled with 64Cu (
METHODS
METHODS
μPET was performed 14 days (
RESULTS
RESULTS
In vivo results showed that Cu-ASTM and Cu-Cl2 accumulated in hypoxic areas of the tumors. Cu-ATSM also stained, to a lesser extent, non-hypoxic regions, such as regions of astrogliosis, with high expression of copper transporters and in particular DMT-1 and CTR1, and also characterized by the expression of elevated astrogliosis. In vitro results show that 64Cu-ATSM showed an increase in the uptake only in severe hypoxia at 0.5 and 0.2% of oxygen while for
CONCLUSION
CONCLUSIONS
In the present study, we show that Cu-complexes undoubtedly accumulate in hypoxic areas of the tumors. This uptake may be the reflection of a direct dependency to a redox metabolism and also a reflection of hypoxic-induced overexpression of transporters. We also show that Cu-ATSM also stained non-hypoxic regions such as astrogliosis.
Identifiants
pubmed: 31858290
doi: 10.1186/s13550-019-0586-6
pii: 10.1186/s13550-019-0586-6
pmc: PMC6923301
doi:
Types de publication
Journal Article
Langues
eng
Pagination
114Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-11-LABEX-0018-01
Organisme : Agence Nationale de la Recherche
ID : ANR-11- LABX-0016-01
Organisme : Agence Nationale de la Recherche
ID : ANR-11-EQPX-0004
Organisme : Agence Nationale de la Recherche
ID : ANR-16-IDEX-0007
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