Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis.
foot-and-mouth disease
monoclonal antibody
sensitivity
solid-phase competitive ELISA
structural protein
type A
Journal
Pathogens (Basel, Switzerland)
ISSN: 2076-0817
Titre abrégé: Pathogens
Pays: Switzerland
ID NLM: 101596317
Informations de publication
Date de publication:
17 Dec 2019
17 Dec 2019
Historique:
received:
12
11
2019
revised:
13
12
2019
accepted:
15
12
2019
entrez:
22
12
2019
pubmed:
22
12
2019
medline:
22
12
2019
Statut:
epublish
Résumé
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O
Identifiants
pubmed: 31861046
pii: pathogens8040301
doi: 10.3390/pathogens8040301
pmc: PMC6963590
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Research Council of Science and Technology
ID : CAP-16-02-KIST
Organisme : Korea Research Institute of Bioscience and Biotechnology
ID : Research Initiative Program
Références
Vet Res. 2001 Jan-Feb;32(1):1-30
pubmed: 11254174
MAbs. 2012 Jul-Aug;4(4):445-57
pubmed: 22684311
Annu Rev Biomed Eng. 2015;17:191-216
pubmed: 26274600
Int J Oncol. 2010 Jun;36(6):1453-9
pubmed: 20428769
Virol Sin. 2011 Aug;26(4):273-8
pubmed: 21847759
Hybridoma (Larchmt). 2012 Oct;31(5):358-63
pubmed: 23098303
Vet Rec. 2007 Feb 17;160(7):238-41
pubmed: 17308024
Transbound Emerg Dis. 2013 Apr;60(2):188-92
pubmed: 22630568
Clin Microbiol Rev. 2004 Apr;17(2):465-93
pubmed: 15084510
BMC Vet Res. 2018 Nov 28;14(1):371
pubmed: 30486820
Mol Cells. 2011 May;31(5):413-21
pubmed: 21448588
Vet Rec. 2012 Apr 7;170(14):350
pubmed: 22492791
Transbound Emerg Dis. 2012 Dec;59(6):476-81
pubmed: 23025522
J Virol Methods. 2001 Sep;97(1-2):33-48
pubmed: 11483215
J Virol Methods. 2004 Feb;115(2):145-58
pubmed: 14667530
J Vet Sci. 2017 Aug 31;18(S1):323-331
pubmed: 28859272
Sci Rep. 2018 Jan 29;8(1):1760
pubmed: 29379029
Vet J. 2004 Jan;167(1):9-22
pubmed: 14623146
Prev Vet Med. 2013 Nov 1;112(3-4):161-73
pubmed: 23958457
PLoS One. 2018 Jun 18;13(6):e0195302
pubmed: 29912868
J Vet Sci. 2018 Nov 30;19(6):788-797
pubmed: 30304889
Vet Res. 2013 Dec 05;44:116
pubmed: 24308718
Virol J. 2016 Nov 28;13(1):195
pubmed: 27894355