Characterization of Antibiotic and Biocide Resistance Genes and Virulence Factors of Staphylococcus Species Associated with Bovine Mastitis in Rwanda.
FTIR spectroscopy
MLST
Staphylococcus aureus
Staphylococcus species
antibiotic resistance
bovine mastitis
capsule serotyping
dru typing
spa typing
whole-genome sequencing
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
18 Dec 2019
18 Dec 2019
Historique:
received:
28
11
2019
revised:
09
12
2019
accepted:
13
12
2019
entrez:
22
12
2019
pubmed:
22
12
2019
medline:
22
12
2019
Statut:
epublish
Résumé
The present study was conducted from July to August 2018 on milk samples taken at dairy farms in the Northern Province and Kigali District of Rwanda in order to identify Staphylococcus spp. associated with bovine intramammary infection. A total of 161 staphylococcal isolates originating from quarter milk samples of 112 crossbred dairy cattle were included in the study. Antimicrobial susceptibility testing was performed and isolates were examined for the presence of various resistance genes. Staphylococcus aureus isolates were also analyzed for the presence of virulence factors, genotyped by spa typing and further phenotypically subtyped for capsule expression using Fourier Transform Infrared (FTIR) spectroscopy. Selected S. aureus were characterized using DNA microarray technology, multi-locus sequence typing (MLST) and whole-genome sequencing. All mecA-positive staphylococci were further genotyped using dru typing. In total, 14 different staphylococcal species were detected, with S. aureus being most prevalent (26.7%), followed by S. xylosus (22.4%) and S. haemolyticus (14.9%). A high number of isolates was resistant to penicillin and tetracycline. Various antimicrobial and biocide resistance genes were detected. Among S. aureus, the Panton-Valentine leukocidin (PVL) genes, as well as bovine leukocidin (LukM/LukF-P83) genes, were detected in two and three isolates, respectively, of which two also carried the toxic shock syndrome toxin gene tsst-1 bovine variant. t1236 was the predominant spa type. FTIR-based capsule serotyping revealed a high prevalence of non-encapsulated S. aureus isolates (89.5%). The majority of the selected S. aureus isolates belonged to clonal complex (CC) 97 which was determined using DNA microarray based assignment. Three new MLST sequence types were detected.
Identifiants
pubmed: 31861266
pii: antibiotics9010001
doi: 10.3390/antibiotics9010001
pmc: PMC7167805
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Austrian Science Fund FWF
ID : P 29304
Pays : Austria
Organisme : Austrian Science Fund
ID : FWF-P29304-B22
Déclaration de conflit d'intérêts
The authors declare no conflict of interest
Références
J Dairy Sci. 2001 Sep;84(9):2018-24
pubmed: 11573781
Microbiol Spectr. 2018 Jul;6(4):
pubmed: 29992898
Trop Anim Health Prod. 2017 Aug;49(6):1101-1106
pubmed: 28526987
J S Afr Vet Assoc. 2015 Jul 14;86(1):1228
pubmed: 26244583
Int J Antimicrob Agents. 2018 Dec;52(6):930-935
pubmed: 30077662
Clin Microbiol Infect. 2011 Oct;17(10):1507-13
pubmed: 21595798
Am J Trop Med Hyg. 2015 Apr;92(4):865-70
pubmed: 25646259
Vet Microbiol. 2016 Aug 15;191:88-95
pubmed: 27374912
J Antimicrob Chemother. 2010 Apr;65(4):619-25
pubmed: 20164198
J Antimicrob Chemother. 1999 Jan;43(1):5-14
pubmed: 10381095
Vet Microbiol. 1998 Oct;63(2-4):217-27
pubmed: 9851000
Clin Microbiol Infect. 2008 Jun;14(6):534-45
pubmed: 18373691
J Clin Microbiol. 2000 Feb;38(2):846-50
pubmed: 10655395
Clin Microbiol Infect. 2008 Oct;14(10):964-9
pubmed: 18828855
Trop Anim Health Prod. 2018 Feb;50(2):259-266
pubmed: 28980098
J Dairy Sci. 2009 Oct;92(10):4962-70
pubmed: 19762813
Oncotarget. 2017 Jul 22;8(35):58086-58097
pubmed: 28938539
Vet Microbiol. 2018 Sep;223:173-180
pubmed: 30173744
J Clin Microbiol. 2006 Dec;44(12):4444-54
pubmed: 17005735
J S Afr Vet Assoc. 2014 Feb 26;85(1):950
pubmed: 24831695
J Glob Antimicrob Resist. 2013 Dec;1(4):189-193
pubmed: 27873611
Clin Microbiol Infect. 2014 Jul;20(7):589-96
pubmed: 24861767
Antimicrob Agents Chemother. 2012 Sep;56(9):4746-52
pubmed: 22733067
Trop Anim Health Prod. 2019 Sep;51(7):2037-2044
pubmed: 31030333
J Clin Microbiol. 2005 Sep;43(9):4363-8
pubmed: 16145078
EMBO Rep. 2013 Jun;14(6):497-501
pubmed: 23681441
Antimicrob Agents Chemother. 2006 Apr;50(4):1156-63
pubmed: 16569824
Vet Microbiol. 2015 Jun 12;177(3-4):353-8
pubmed: 25891423
Ann Clin Microbiol Antimicrob. 2016 Sep 20;15(1):51
pubmed: 27647271
Syst Appl Microbiol. 2016 May;39(3):189-194
pubmed: 27021524
Vet Res. 2012 May 15;43:42
pubmed: 22587484
BMC Infect Dis. 2017 Jun 13;17(1):422
pubmed: 28610560
J Dairy Sci. 2015 Sep;98(9):6256-69
pubmed: 26188567
Front Cell Infect Microbiol. 2017 Apr 19;7:127
pubmed: 28469994
Vet Microbiol. 2009 Feb 16;134(1-2):3-8
pubmed: 18848410
J Antimicrob Chemother. 2014 Apr;69(4):919-23
pubmed: 24324222
Front Microbiol. 2016 Oct 13;7:1603
pubmed: 27790200
Vet Microbiol. 1997 Dec;59(1):53-8
pubmed: 9460196
Braz J Microbiol. 2017 Jan - Mar;48(1):159-166
pubmed: 27889420
Vet Microbiol. 2014 Dec 5;174(3-4):489-495
pubmed: 25448449
Biosci Biotechnol Biochem. 2004 May;68(5):981-1003
pubmed: 15170101
Antimicrob Agents Chemother. 2002 Sep;46(9):2797-803
pubmed: 12183231
Trop Anim Health Prod. 2014 Jan;46(1):99-105
pubmed: 23955012
J Dairy Res. 1997 Feb;64(1):23-37
pubmed: 9120075
Eur J Clin Microbiol Infect Dis. 2017 Jul;36(7):1163-1171
pubmed: 28116552
J Dairy Sci. 2016 Jan;99(1):515-28
pubmed: 26601578
J Clin Microbiol. 2014 Jul;52(7):2365-70
pubmed: 24759713
Front Microbiol. 2015 Oct 20;6:1160
pubmed: 26539185
Future Microbiol. 2010 Dec;5(12):1823-32
pubmed: 21155664
Microb Drug Resist. 2007 Summer;13(2):77-84
pubmed: 17650957
Vet J. 2015 Jan;203(1):44-51
pubmed: 25467994
Genome Announc. 2017 Oct 19;5(42):
pubmed: 29051260
J Vet Med. 2017;2017:8456713
pubmed: 28798952
Res Vet Sci. 2016 Apr;105:192-4
pubmed: 27033931
Front Microbiol. 2017 Apr 06;8:511
pubmed: 28428772
J Clin Microbiol. 2010 Jun;48(6):2130-9
pubmed: 20392913
J Clin Microbiol. 2013 Jul;51(7):2261-6
pubmed: 23658268
PLoS One. 2013 Jun 20;8(6):e66153
pubmed: 23840416
J Antimicrob Chemother. 2004 Nov;54(5):936-9
pubmed: 15471995
Antimicrob Agents Chemother. 1999 May;43(5):1062-6
pubmed: 10223914
BMC Vet Res. 2018 Aug 23;14(1):246
pubmed: 30139356
Antimicrob Agents Chemother. 2014 Oct;58(10):6133-8
pubmed: 25092709
Vet Microbiol. 2014 Jan 31;168(2-4):381-7
pubmed: 24332703
Food Microbiol. 2017 Aug;65:64-73
pubmed: 28400021
Sci Rep. 2018 Oct 29;8(1):15968
pubmed: 30374136
J S Afr Vet Assoc. 2017 Jun 21;88(0):e1-e6
pubmed: 28697611
Antimicrob Agents Chemother. 2000 Feb;44(2):231-8
pubmed: 10639342
Appl Environ Microbiol. 2011 May;77(9):3052-60
pubmed: 21378035
J Antimicrob Chemother. 2013 Jun;68(6):1251-5
pubmed: 23386262
Trop Anim Health Prod. 2017 Dec;49(8):1645-1656
pubmed: 28823069
Sci Rep. 2016 Nov 25;6:37759
pubmed: 27886237
Antimicrob Agents Chemother. 2015 Nov 09;60(1):678-81
pubmed: 26552979
Emerg Infect Dis. 2006 Feb;12(2):333-6
pubmed: 16494767
J Dairy Sci. 2004 Aug;87(8):2433-41
pubmed: 15328265
Sci Rep. 2018 Jun 21;8(1):9467
pubmed: 29930324