Human Polyomaviruses in the Cerebrospinal Fluid of Neurological Patients.
cerebrospinal fluid
human polyomaviruses
neurological diseases
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
20 Dec 2019
20 Dec 2019
Historique:
received:
23
10
2019
revised:
06
12
2019
accepted:
17
12
2019
entrez:
22
12
2019
pubmed:
22
12
2019
medline:
22
12
2019
Statut:
epublish
Résumé
Central nervous system (CNS) infections by human polyomaviruses (HPyVs), with the exception of JC (JCPyV), have been poorly studied. In total, 234 cerebrospinal fluid (CSF) samples were collected from patients affected with neurological disorders. DNA was isolated and subjected to quantitative real-time PCR (Q-PCR) for the detection of six HPyVs: JCPyV, BKPyV, Merkel cell PyV (MCPyV), HPyV6, HPyV7, and HPyV9. Where possible, the molecular characterization of the viral strains was carried out by nested PCR and automated sequencing. JCPyV was detected in 3/234 (1.3%), BKPyV in 15/234 (6.4%), MCPyV in 22/234 (9.4%), and HPyV6 in 1/234 (0.4%) CSF samples. JCPyV was detected at the highest ( HPyVs other than JCPyV were found in the CSF of patients affected with different neurological diseases, probably as bystanders, rather than etiological agents of the disease. However, the fact that they can be latent in the CNS should be considered, especially in immunosuppressed patients.
Sections du résumé
BACKGROUND
BACKGROUND
Central nervous system (CNS) infections by human polyomaviruses (HPyVs), with the exception of JC (JCPyV), have been poorly studied.
METHODS
METHODS
In total, 234 cerebrospinal fluid (CSF) samples were collected from patients affected with neurological disorders. DNA was isolated and subjected to quantitative real-time PCR (Q-PCR) for the detection of six HPyVs: JCPyV, BKPyV, Merkel cell PyV (MCPyV), HPyV6, HPyV7, and HPyV9. Where possible, the molecular characterization of the viral strains was carried out by nested PCR and automated sequencing.
RESULTS
RESULTS
JCPyV was detected in 3/234 (1.3%), BKPyV in 15/234 (6.4%), MCPyV in 22/234 (9.4%), and HPyV6 in 1/234 (0.4%) CSF samples. JCPyV was detected at the highest (
CONCLUSIONS
CONCLUSIONS
HPyVs other than JCPyV were found in the CSF of patients affected with different neurological diseases, probably as bystanders, rather than etiological agents of the disease. However, the fact that they can be latent in the CNS should be considered, especially in immunosuppressed patients.
Identifiants
pubmed: 31861837
pii: microorganisms8010016
doi: 10.3390/microorganisms8010016
pmc: PMC7022863
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : PRIN2015 2015w729wh
Organisme : Università degli Studi di Milano
ID : PSR 2017 -PSR 2018
Références
PLoS One. 2011 Mar 16;6(3):e16736
pubmed: 21436884
Virology. 2017 Jun;506:45-54
pubmed: 28342387
J Clin Virol. 2009 Jul;45(3):249-54
pubmed: 19515607
J Virol. 2012 Oct;86(19):10887
pubmed: 22966183
Emerg Infect Dis. 2012 Oct;18(10):1676-9
pubmed: 23017293
PLoS One. 2012;7(11):e49449
pubmed: 23166671
Virology. 2013 Feb 20;436(2):295-303
pubmed: 23276405
J Neurovirol. 2001 Aug;7(4):280-7
pubmed: 11517403
J Infect Dis. 2009 Jul 15;200(2):314-5
pubmed: 19530941
Vox Sang. 2004 Apr;86(3):178-82
pubmed: 15078252
J Virol. 2012 Oct;86(19):10321-6
pubmed: 22740408
J Infect Dis. 2014 Nov 15;210(10):1595-9
pubmed: 24795478
J Clin Microbiol. 1996 Jan;34(1):159-64
pubmed: 8748293
PLoS One. 2018 Oct 23;13(10):e0206273
pubmed: 30352098
J Virol. 2009 Nov;83(21):10846-56
pubmed: 19640999
J Infect Dis. 2015 May 15;211(10):1560-5
pubmed: 25231015
Front Oncol. 2019 Aug 08;9:711
pubmed: 31440465
J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Jun 1;12(2):139-46
pubmed: 8680884
Virology. 1992 Nov;191(1):72-80
pubmed: 1329338
J Infect Dis. 2009 Dec 1;200(11):1755-8
pubmed: 19860559
PLoS One. 2011;6(7):e22468
pubmed: 21799863
PLoS Pathog. 2010 Jul 29;6(7):e1001024
pubmed: 20686659
Science. 2008 Feb 22;319(5866):1096-100
pubmed: 18202256
Eur J Haematol. 2017 May;98(5):450-458
pubmed: 28129450
Clin Nephrol. 2019 Feb;91(2):107-113
pubmed: 30526816
Ann Pharmacother. 2013 Sep;47(9):1229-33
pubmed: 24259742
J Virol. 2007 Apr;81(8):4130-6
pubmed: 17287263
PLoS One. 2013 May 08;8(5):e62764
pubmed: 23667518
N Engl J Med. 2002 Aug 15;347(7):488-96
pubmed: 12181403
J Infect Dis. 2018 Apr 23;217(10):1601-1611
pubmed: 29409030
Am J Hum Genet. 1998 Apr;62(4):768-75
pubmed: 9529358
Transpl Infect Dis. 2016 Dec;18(6):950-953
pubmed: 27696719
J Med Virol. 2015 Jul;87(7):1241-7
pubmed: 25754536
J Virol. 2011 May;85(9):4586-90
pubmed: 21307194
Emerg Infect Dis. 2009 Mar;15(3):489-91
pubmed: 19239773
Lancet. 1971 Jun 19;1(7712):1257-60
pubmed: 4104715
N Engl J Med. 1986 Jul 24;315(4):230-4
pubmed: 3014334
J Neurovirol. 2005 Feb;11(1):51-7
pubmed: 15804959
Anticancer Res. 2011 Oct;31(10):3489-92
pubmed: 21965766
Virus Res. 2010 May;149(2):190-6
pubmed: 20138933
J Gen Virol. 1993 Aug;74 ( Pt 8):1499-507
pubmed: 8393910
J Med Virol. 2012 Sep;84(9):1464-70
pubmed: 22825826
J Cell Physiol. 2012 Jan;227(1):136-45
pubmed: 21374594
Transfusion. 2001 Jan;41(1):74-81
pubmed: 11161249
J Am Acad Dermatol. 2017 May;76(5):932-940.e3
pubmed: 28040372
Cell Host Microbe. 2010 Jun 25;7(6):509-15
pubmed: 20542254
PLoS One. 2013;8(3):e58021
pubmed: 23516426
J Clin Virol. 2015 Jul;68:24-7
pubmed: 26071330
J Gen Virol. 2013 Mar;94(Pt 3):482-496
pubmed: 23255626
J Med Virol. 1993 Jan;39(1):50-6
pubmed: 7678637
PLoS Pathog. 2007 May 4;3(5):e64
pubmed: 17480120