A predation assay using amoebae to screen for virulence factors unearthed the first W. chondrophila inclusion membrane protein.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 12 2019
20 12 2019
Historique:
received:
08
04
2019
accepted:
11
11
2019
entrez:
22
12
2019
pubmed:
22
12
2019
medline:
11
11
2020
Statut:
epublish
Résumé
Waddlia chondrophila is an intracellular bacterium phylogenetically related to the well-studied human and animal pathogens of the Chlamydiaceae family. In the last decade, W. chondrophila was convincingly demonstrated to be associated with adverse pregnancy outcomes in humans and abortions in animals. All members of the phylum Chlamydiae possess a Type Three Secretion System that they use for delivering virulence proteins into the host cell cytosol to modulate their environment and create optimal conditions to complete their life cycle. To identify W. chondrophila virulence proteins, we used an original screening approach that combines a cosmid library with an assay monitoring resistance to predation by phagocytic amoebae. This technique combined with bioinformatic data allowed the identification of 28 candidate virulence proteins, including Wimp1, the first identified inclusion membrane protein of W. chondrophila.
Identifiants
pubmed: 31862969
doi: 10.1038/s41598-019-55511-1
pii: 10.1038/s41598-019-55511-1
pmc: PMC6925127
doi:
Substances chimiques
Membrane Proteins
0
Type III Secretion Systems
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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