Environmental conditions steer phenotypic switching in acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus, affecting PirA
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
21
10
2019
revised:
12
12
2019
accepted:
18
12
2019
pubmed:
24
12
2019
medline:
6
3
2021
entrez:
24
12
2019
Statut:
ppublish
Résumé
Bacteria in nature are widely exposed to differential fluid shears which are often a trigger for phenotypic switches. The latter mediates transcriptional and translation remodelling of cellular metabolism impacting among others virulence, antimicrobial resistance and stress resistance. In this study, we evaluated the role of fluid shear on phenotypic switch in an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus M0904 strain under both in vitro and in vivo conditions. The results showed that V. parahaemolyticus M0904 grown at lower shaking speed (110 rpm constant agitation, M0904/110), causing low fluid shear, develop cellular aggregates or floccules. These cells increased levan production (as verified by concanavalin binding) and developed differentially stained colonies on Congo red agar plates and resistance to antibiotics. In addition, the phenotypic switch causes a major shift in the protein secretome. At 120 rpm (M0904/120), PirA
Identifiants
pubmed: 31867836
doi: 10.1111/1462-2920.14903
doi:
Substances chimiques
Bacterial Toxins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4212-4230Subventions
Organisme : Hercules
ID : 20140910-03
Pays : International
Organisme : EMBRC Belgium - FWO
ID : 20151029-03
Pays : International
Organisme : Special Research Fund of Ghent University
ID : BOF12/BAS/042
Pays : International
Organisme : Netaji Subhas International Doctoral Fellowship from Indian Council of Agricultural Research (ICAR), New Delhi
Pays : International
Informations de copyright
© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.
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