Piezo channel plays a part in retinal ganglion cell damage.
Animals
Blotting, Western
Cells, Cultured
Cornea
/ metabolism
Ependymoglial Cells
Epithelial Cells
/ metabolism
Immunohistochemistry
Intercellular Signaling Peptides and Proteins
/ pharmacology
Intraocular Pressure
Ion Channels
/ agonists
Lens, Crystalline
/ metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Neuronal Outgrowth
/ physiology
Ocular Hypertension
/ etiology
Pyrazines
/ pharmacology
Rats
Rats, Sprague-Dawley
Retinal Diseases
/ etiology
Retinal Ganglion Cells
/ drug effects
Spider Venoms
/ pharmacology
Thiadiazoles
/ pharmacology
Trabecular Meshwork
/ metabolism
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
18
09
2019
revised:
27
11
2019
accepted:
20
12
2019
pubmed:
25
12
2019
medline:
25
7
2020
entrez:
25
12
2019
Statut:
ppublish
Résumé
Piezo channel is one of the mechanosensitive channels that senses pressure and shearing stress. Previous reports show that Piezo channel is expressed in many tissues such as skin and lung and they have many important roles. In addition, the mRNA of Piezo has been detected in astrocytes in the optic nerve head of mice. However, it is not yet clear where Piezo channel localize in eye and what kind of effects it have. Thus, the purpose of this study was to determine the expression sites of Piezo channel in mouse eyes and effect of Piezo channel on retinal ganglion cells. Immunostaining analysis showed that the Piezo 1/2 were expressed in the cornea, trabecular meshwork of the anterior ocular segment, lens epithelial cells, and on the retinal ganglion cell layer. The expression of retinal Piezo 2 was increased in retinal disorder model mouse caused by high IOP. Piezo 1 agonist Yoda 1 suppressed neurite outgrowth in retinal ganglion cells. On the other hand, Piezo antagonist GsMTx4 promoted neurite outgrowth in retinal ganglion cells. These findings indicate that Piezo channel may contribute to diseases relating the IOP such as glaucoma.
Identifiants
pubmed: 31874142
pii: S0014-4835(19)30703-1
doi: 10.1016/j.exer.2019.107900
pii:
doi:
Substances chimiques
Intercellular Signaling Peptides and Proteins
0
Ion Channels
0
MTx4 protein, Grammostola spatulata
0
Piezo1 protein, mouse
0
Piezo2 protein, mouse
0
Pyrazines
0
Spider Venoms
0
Thiadiazoles
0
yoda-1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107900Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.