Spinal cord stimulation prevents paclitaxel-induced mechanical and cold hypersensitivity and modulates spinal gene expression in rats.
Chemotherapy-induced peripheral neuropathy
Neuropathic pain
RNA-sequencing
Rat
Spinal cord stimulation
Journal
Pain reports
ISSN: 2471-2531
Titre abrégé: Pain Rep
Pays: United States
ID NLM: 101683899
Informations de publication
Date de publication:
Historique:
received:
25
04
2019
revised:
06
08
2019
accepted:
10
08
2019
entrez:
26
12
2019
pubmed:
26
12
2019
medline:
26
12
2019
Statut:
epublish
Résumé
Paclitaxel-induced peripheral neuropathy (PIPN) is a common dose-limiting side effect of this cancer treatment drug. Spinal cord stimulation (SCS) has demonstrated efficacy for attenuating some neuropathic pain conditions. We aim to examine the inhibitory effect of SCS on the development of PIPN pain and changes of gene expression in the spinal cord in male rats after SCS. We examined whether traditional SCS (50 Hz, 6-8 h/session daily for 14 consecutive days) administered during paclitaxel treatment (1.5 mg/kg, i.p.) attenuates PIPN-related pain behavior. After SCS treatment, we performed RNA-seq of the lumbar spinal cord to examine which genes are differentially expressed after PIPN with and without SCS. Compared to rats treated with paclitaxel alone (n = 7) or sham SCS (n = 6), SCS treatment (n = 11) significantly inhibited the development of paclitaxel-induced mechanical and cold hypersensitivity, without altering open-field exploratory behavior. RNA-seq showed that SCS induced upregulation of 836 genes and downregulation of 230 genes in the spinal cord of paclitaxel-treated rats (n = 3) as compared to sham SCS (n = 5). Spinal cord stimulation upregulated immune responses in paclitaxel-treated rats, including transcription of astrocyte- and microglial-related genes, but repressed transcription of multiple gene networks associated with synapse transmission, neuron projection development, γ-aminobutyric acid reuptake, and neuronal plasticity. Our findings suggest that traditional SCS may attenuate the development of pain-related behaviors in PIPN rats, possibly by causing aggregate inhibition of synaptic plasticity through upregulation and downregulation of gene networks in the spinal cord.
Identifiants
pubmed: 31875188
doi: 10.1097/PR9.0000000000000785
pii: PAINREPORTS-D-19-0076
pmc: PMC6882571
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e785Subventions
Organisme : NINDS NIH HHS
ID : R01 NS070814
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS110598
Pays : United States
Informations de copyright
Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The International Association for the Study of Pain.
Déclaration de conflit d'intérêts
Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article.
Références
J Med Genet. 2013 Sep;50(9):599-605
pubmed: 23776197
Pain. 2014 Dec;155(12):2461-70
pubmed: 25261162
Reg Anesth Pain Med. 2016 Nov/Dec;41(6):750-756
pubmed: 27512935
Am J Physiol Heart Circ Physiol. 2007 Jan;292(1):H311-7
pubmed: 16920800
Anesthesiology. 2018 Jun;128(6):1220-1236
pubmed: 29601322
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11849-53
pubmed: 7527545
Pain. 2011 Jul;152(7):1666-73
pubmed: 21514998
Curr Opin Support Palliat Care. 2016 Jun;10(2):119-128
pubmed: 27054288
PLoS One. 2012;7(6):e39765
pubmed: 22745825
Cancer Med. 2016 Apr;5(4):631-9
pubmed: 26763541
Pain. 1996 Aug;66(2-3):287-95
pubmed: 8880852
Oncology (Williston Park). 2016 Nov 15;30(11):1020-9
pubmed: 27854104
Genome Biol. 2014;15(12):550
pubmed: 25516281
Eur J Pain. 2017 Mar;21(3):507-519
pubmed: 27714945
Pain. 1997 Oct;73(1):87-95
pubmed: 9414060
J Neuroimmunol. 2010 Dec 15;229(1-2):26-50
pubmed: 20870295
Neurosurgery. 1995 Aug;37(2):266-71; discussion 271-2
pubmed: 7477778
Pain. 2016 Nov;157(11):2582-2593
pubmed: 27589093
Brain Res. 2011 Aug 8;1405:95-108
pubmed: 21741621
J Pain. 2013 Oct;14(10):1031-44
pubmed: 23726937
Brain Behav Immun. 2015 Oct;49:255-66
pubmed: 26065826
Anesthesiology. 2010 Dec;113(6):1392-405
pubmed: 21068658
Anesthesiology. 2019 Apr;130(4):651-665
pubmed: 30556812
Neurosci Lett. 1997 Feb 28;223(3):173-6
pubmed: 9080460
J Pain. 2014 Jul;15(7):712-25
pubmed: 24755282
Int J Endocrinol. 2014;2014:674987
pubmed: 24883061
Exp Neurol. 2016 Sep;283(Pt B):550-9
pubmed: 27151600
Pain Pract. 2014 Nov;14(8):709-20
pubmed: 24165268
Neuroscience. 2012 Jul 26;215:196-208
pubmed: 22548781
Support Care Cancer. 2014 May;22(5):1161-9
pubmed: 24362842
Nat Rev Neurosci. 2016 Jun;17(6):337-50
pubmed: 27080385
Science. 2000 Jun 9;288(5472):1765-9
pubmed: 10846153
Neuromodulation. 2017 Aug;20(6):525-533
pubmed: 28568898
Mol Pain. 2018 Jan-Dec;14:1744806918817429
pubmed: 30451078
J Neurosci. 2019 Jan 16;39(3):393-411
pubmed: 30459229
J Pain. 2016 Jul;17(7):775-86
pubmed: 26979998
PLoS One. 2017 Jan 26;12(1):e0170814
pubmed: 28125674
Nat Rev Neurosci. 2012 Feb 15;13(3):169-82
pubmed: 22334212
Nat Neurosci. 2007 Nov;10(11):1361-8
pubmed: 17965656
Pain. 2014 Dec;155(12):2560-7
pubmed: 25242567
J Neurosci. 2011 Aug 10;31(32):11547-52
pubmed: 21832185
Anesthesiology. 2013 Aug;119(2):422-32
pubmed: 23880991
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Nucleic Acids Res. 2009 Jan;37(Database issue):D680-5
pubmed: 18948278
Cancer. 2015 Oct 1;121(19):3507-14
pubmed: 26207518
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Annu Rev Pharmacol Toxicol. 1980;20:441-62
pubmed: 7387124
Eur J Pain. 2011 Feb;15(2):111-7
pubmed: 20591705
Plast Reconstr Surg. 1999 Apr;103(4):1221-8
pubmed: 10088510
Neuroscience. 2011 Oct 13;193:440-51
pubmed: 21763756
J Neurol Neurosurg Psychiatry. 2018 Jun;89(6):636-641
pubmed: 29439162
Neuron. 2004 Nov 18;44(4):637-50
pubmed: 15541312
J Oncol Pract. 2014 Nov;10(6):e421-e424
pubmed: 29424607
Pain Pract. 2011 Mar-Apr;11(2):148-53
pubmed: 21371254
Brain Res. 2003 May 23;973(1):39-43
pubmed: 12729951
J Biochem. 2016 May;159(5):491-6
pubmed: 26861995
Curr Pain Headache Rep. 2013 Oct;17(10):364
pubmed: 23996720
Neuroscience. 2006 Dec 1;143(2):541-6
pubmed: 16978792
Pain. 2001 Dec;94(3):293-304
pubmed: 11731066
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W305-11
pubmed: 19465376
J Pain Symptom Manage. 2004 Jan;27(1):72-8
pubmed: 14711471