Differences in Stability of Viral and Viral-Cellular Fusion Transcripts in HPV-Induced Cervical Cancers.
HPV mRNA stability
cervical cancer
viral-cellular fusion transcripts
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
23 Dec 2019
23 Dec 2019
Historique:
received:
10
11
2019
revised:
18
12
2019
accepted:
19
12
2019
entrez:
28
12
2019
pubmed:
28
12
2019
medline:
8
5
2020
Statut:
epublish
Résumé
HPV-DNA integration results in dysregulation of viral oncogene expression. Because viral-cellular fusion transcripts inherently lack the viral AU-rich elements of the 3'UTR, they are considered to be more stable than episome-derived transcripts. The aim of this study is to provide formal proof for this assumption by comparing the stability of viral early transcripts derived from episomal and integrated HPV16 DNA, respectively. Full-length cDNA of three fusion transcripts comprising viral and cellular sequences in sense orientation were amplified and cloned into the adeno-viral-vector pAd/CMV/V5-DEST. The most abundant HPV16 oncogene transcript E6*I
Identifiants
pubmed: 31877944
pii: ijms21010112
doi: 10.3390/ijms21010112
pmc: PMC6981427
pii:
doi:
Substances chimiques
E6 protein, Human papillomavirus type 16
0
Oncogene Proteins, Viral
0
RNA, Viral
0
Repressor Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Universitätsklinikum Jena
ID : LoM2015-2018
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