Cell-Type-Specific Quantification of a Scaffold-Based 3D Liver Co-Culture.
3D culture
PCR-based method
cell number
co-culture
quantification
Journal
Methods and protocols
ISSN: 2409-9279
Titre abrégé: Methods Protoc
Pays: Switzerland
ID NLM: 101720073
Informations de publication
Date de publication:
23 Dec 2019
23 Dec 2019
Historique:
received:
13
06
2019
revised:
18
12
2019
accepted:
18
12
2019
entrez:
28
12
2019
pubmed:
28
12
2019
medline:
28
12
2019
Statut:
epublish
Résumé
In order to increase the metabolic activity of human hepatocytes and liver cancer cell lines, many approaches have been reported in recent years. The metabolic activity could be increased mainly by cultivating the cells in 3D systems or co-cultures (with other cell lines). However, if the system becomes more complex, it gets more difficult to quantify the number of cells (e.g., on a 3D matrix). Until now, it has been impossible to quantify different cell types individually in 3D co-culture systems. Therefore, we developed a PCR-based method that allows the quantification of HepG2 cells and 3T3-J2 cells separately in a 3D scaffold culture. Moreover, our results show that this method allows better comparability between 2D and 3D cultures in comparison to the often-used approaches based on metabolic activity measurements, such as the conversion of resazurin.
Identifiants
pubmed: 31878071
pii: mps3010001
doi: 10.3390/mps3010001
pmc: PMC7189675
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : the Federal Ministry for Economic Affairs and Energy within the framework of the ZIM program
ID : ZF 4301401CS6, ZF4301001CS6
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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