Standardized monitoring of cytomegalovirus-specific immunity can improve risk stratification of recurrent cytomegalovirus reactivation after hematopoietic stem cell transplantation.


Journal

Haematologica
ISSN: 1592-8721
Titre abrégé: Haematologica
Pays: Italy
ID NLM: 0417435

Informations de publication

Date de publication:
01 02 2021
Historique:
received: 10 06 2019
accepted: 18 12 2019
pubmed: 28 12 2019
medline: 28 5 2021
entrez: 28 12 2019
Statut: epublish

Résumé

Recurrence of cytomegalovirus reactivation remains a major cause of morbidity and mortality following allogeneic hematopoietic stem cell transplantation. Monitoring cytomegalovirus-specific cellular immunity using a standardized assay might improve the risk stratification of patients. A prospective multicenter study was conducted in 175 intermediate- and high-risk allogeneic hematopoietic stem cell transplant recipients under preemptive antiviral therapy. Cytomegalovirus-specific cellular immunity was measured using a standardized IFN-γ ELISpot assay (T-Track® CMV). Primary aim was to evaluate the suitability of measuring cytomegalovirus-specific immunity after end of treatment for a first cytomegalovirus reactivation to predict recurrent reactivation. 40/101 (39.6%) patients with a first cytomegalovirus reactivation experienced recurrent reactivations, mainly in the high-risk group (cytomegalovirus-seronegative donor/cytomegalovirus-seropositive recipient). The positive predictive value of T-Track® CMV (patients with a negative test after the first reactivation experienced at least one recurrent reactivation) was 84.2% in high-risk patients. Kaplan-Meier analysis revealed a higher probability of recurrent cytomegalovirus reactivation in high-risk patients with a negative test after the first reactivation (hazard ratio 2.73; p=0.007). Interestingly, a post-hoc analysis considering T-Track® CMV measurements at day 100 post-transplantation, a time point highly relevant for outpatient care, showed a positive predictive value of 90.0% in high-risk patients. Our results indicate that standardized cytomegalovirus-specific cellular immunity monitoring may allow improved risk stratification and management of recurrent cytomegalovirus reactivation after hematopoietic stem cell transplantation. This study was registered at www.clinicaltrials.gov as #NCT02156479.

Identifiants

pubmed: 31879324
pii: haematol.2019.229252
doi: 10.3324/haematol.2019.229252
pmc: PMC7849569
doi:

Banques de données

ClinicalTrials.gov
['NCT02156479']

Types de publication

Journal Article Multicenter Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

363-374

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Auteurs

Eva Wagner-Drouet (E)

Dpt of Hematology, Medical Oncology, and Pneumology, University Medical Center, Mainz, Germany.

Daniel Teschner (D)

Dpt of Hematology, Medical Oncology, and Pneumology, University Medical Center, Mainz, Germany.

Christine Wolschke (C)

Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Germany.

Dietlinde Janson (D)

Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Germany.

Kerstin Schäfer-Eckart (K)

Oncology, Hematology and Bone Marrow Transplantation Unit, Klinikum Nord, Nürnberg, Germany.

Johannes Gärtner (J)

Oncology, Hematology and Bone Marrow Transplantation Unit, Klinikum Nord, Nürnberg, Germany.

Stephan Mielke (S)

Department of Laboratory Medicine, Karolinska Institutet and University Hospital, Stockholm, Sweden.

Martin Schreder (M)

First Department of Medicine, Center for Oncology and Hematology, Wilhelminenspital, Vienna, Austria.

Guido Kobbe (G)

Department of Hematology, University Hospital, Heinrich Heine University Düsseldorf, Germany.

Mustafa Kondakci (M)

Department of Hematology, University Hospital, Heinrich Heine University Düsseldorf, Germany.

Inken Hilgendorf (I)

Klinik f. Innere Medizin II, Abt. Haematol. und Internist. Onkologie, Univ.-Klinikum Jena, Germany.

Marie von Lilienfeld-Toal (M)

Klinik f. Innere Medizin II, Abt. Haemmatol. und Internist. Onkologie, Univ.-Klinikum Jena, Germany.

Stefan Klein (S)

Dpt of Hematology and Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim,Germany.

Daniela Heidenreich (D)

Dpt of Hematology and Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim,Germany.

Sebastian Kreil (S)

Dpt of Hematology and Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim,Germany.

Mareike Verbeek (M)

III. Medical Department, Hematology and Oncology, Klinikum rechts der Isar, TUM, Munich, Germany.

Sandra Grass (S)

III. Medical Department, Hematology and Oncology, Klinikum rechts der Isar, TUM, Munich, Germany.

Markus Ditschkowski (M)

Innere Klinik, Tumorforschung, University Hospital Essen, Germany.

Tanja Gromke (T)

Innere Klinik, Tumorforschung, University Hospital Essen, Germany.

Martina Koch (M)

Dpt of Transplantation Surgery, University Medical Center of the JGU, Mainz, Germany.

Monika Lindemann (M)

Institute for Transfusion Medicine, University Hospital Essen, Germany.

Thomas Hünig (T)

Institute of Virology and Immunobiology, University Medical Center Würzburg, Germany.

Traudel Schmidt (T)

Lophius Biosciences, Regensburg, Germany.

Anne Rascle (A)

Lophius Biosciences, Regensburg, Germany.

Harald Guldan (H)

Lophius Biosciences, Regensburg, Germany.

Sascha Barabas (S)

Lophius Biosciences, Regensburg, Germany.

Ludwig Deml (L)

Lophius Biosciences, Regensburg, Germany.

Ralf Wagner (R)

Institute of Clinical Microbiology and Hygiene, University Medical Center Regensburg, Germany.

Daniel Wolff (D)

Dpt of Internal Medicine III, Hematology and Oncology, University Medical Center Regensburg, Germany.

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