Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes.
Actin
Chromosomes
Eggs
Fertility
High-resolution live microscopy
Meiosis
Microtubules
Oocytes
Spindle
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
12
2019
pubmed:
28
12
2019
medline:
20
1
2021
Statut:
ppublish
Résumé
Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter.
Identifiants
pubmed: 31879910
doi: 10.1007/978-1-0716-0219-5_17
pmc: PMC9703194
doi:
Substances chimiques
Actins
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
267-295Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
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